Production of Biosurfactant in 2L Bioreactor Using Sludge Palm Oil as a Substrate

Authors

  • P. JAMAL Bioenvironmental Engineering Research Unit (BERU), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), P.O. BOX 10, 50728 Kuala Lumpur, Malaysia.
  • Md Zahangir Alam
  • E. A. Zainuddin
  • and W. M. F. W. Nawawi

DOI:

https://doi.org/10.31436/iiumej.v12i4.212

Abstract

In this study, sludge palm oil was utilized as a raw material for process optimization in a stirred tank bioreactor for the biosurfactant production. Optimized parameters include temperature, agitation and aeration while the pH and media compositions such as NaNO3, NaCl, FeSO4, Meat extract, and glucose have been fixed from the previous study. The design of this research was made by using the Design-Expert software (2 level factorial design). Surface tension was used as indirect indicator for biosurfactant production. After model validation, yield of biosurfactant was found to be highest when surface tension was at its lowest value (<24 mN/m) at temperature of 300C , agitation 300 rpm and around 0.5 vvm of aeration with percentage error less than 10% between observed value and predicted value. The finding in this research can be applied to produce biosurfactant in large amount from the less expensive material such as sludge palm oil by using the same optimized model equation.


ABSTRAK: Dalam kajian ini, enapcemar kelapa sawit digunakan sebagai bahan mentah untuk pengoptimuman proses dalam bioreaktor bagi penghasilan biosurfactant. Parameter yang dioptimumkan termasuk suhu, pergolakan dan pengudaraan manakala pH dan komposisi media seperti NaNO3, NaCl, FeSO4, ekstrak daging, dan glukos telah ditetapkan daripada kajian sebelumnya. Reka bentuk kajian ini telah dilakukan dengan menggunakan perisian Design Expert (2 Level factorial design). Tegangan permukaan digunakan sebagai penunjuk tidak langsung untuk penghasilan biosurfactant. Selepas pengesahan, hasil biosurfactant didapati terbanyak apabila tegangan permukaan pada nilai terendah (<24 mN/m) pada suhu 300C, pergolakan 300 rpm, dan sekitar 0.5 vvm pengudaraan dengan margin perbezaan kurang daripada 10% antara nilai sebenar cerapan dan nilai yang diramalkan. Penemuan dalam kajian ini boleh digunakan untuk menghasilkan biosurfactant dalam jumlah yang besar dari bahan yang murah seperti enapcemar kelapa sawit dengan menggunakan model optimum yang sama.

KEY WORDS:  Biosurfactant, Sludge Palm Oil, Process optimization, and Factorial design.

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How to Cite

JAMAL, P., Alam, M. Z., Zainuddin, E. A., & Nawawi, and W. M. F. W. (2011). Production of Biosurfactant in 2L Bioreactor Using Sludge Palm Oil as a Substrate. IIUM Engineering Journal, 12(4). https://doi.org/10.31436/iiumej.v12i4.212