Preliminary Investigation of Myo-Inositol Phosphates Produced by ASUIA279 Phytase on MCF-7 Cancer Cells

  • N. Mohd. Yusoff Bioprocess and Molecular Engineering Research Unit, Deaprtment of Biotechnology Engineering, International Islamic University Malaysia (IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia.
  • T. Nuge
  • N.H. Zainan
  • Y.Z.H-Y. Hashim
  • P. Jamal
  • Anis Shobirin Meor Hussin
  • Abd-Elaziem Farouk
  • and H.M. Salleh


Phytate or myo-inositol hexakisphosphates (IP6) is widely distributed in plants like rice brans. The production of myo-inositol phosphate intermediates has received much attention due to the remarkable potential health benefits offered by the compounds. In this study, the cytotoxicity of the partially purified myo-inositol phosphate fractions and commercial IP1 and IP6 were investigated against MCF-7 breast cancer cell lines. The study showed that the commercial standard IP1 and IP6 showed good inhibition towards the MCF-7 cell line. The MCF-7 cells growth was inhibited in minimum concentration of myo-inositol phosphates (<1000 µg/ml). However, no inhibition observed on the MCF-7 cell line by the myo-inositol phosphates fractions partially purified from rice bran at concentration <1000 ?g/ml. The inhibition of MCF-7 was only observed at concentration more than 30 mg/ml with more than 40% cells were inhibited. This indicates that the partially purified rice bran myo-inositol phosphates degraded by ASUIA279 phytase on MCF-7 breast cancer cells exhibit positive results towards the inhibition of cancer cells growth at relatively high concentration..

KEYWORDS: myo-inositol phosphates, phytase, MCF-7,  cancer

ABSTRAK: Fitat atau myo-inositol hexakisphosphate (IP6) dikenali umum teragih di dalam tumbuhan seperti dedak padi. Penghasilan perantaraan fosfat myo-inositol mendapat perhatian memandangkan ia berpotensi tinggi dalam kesihatan. Dalam kajian ini, kesitotoksikan sebahagian daripada fosfat myo-inositol separa tulen, IP1 komersil dan IP6 komersil dikaji terhadap produk yang berupa sel kekal (cell lines) kanser payu dara MCF-7. Tumbesaran sel MCF-7 direncatkan dalam pekatan minima fosfat myo-inositol (<1000 μg/ml). Tetapi, tidak ada perencatan dilihat terhadap sel kekal MCF-7 oleh sebahagian fosfat myo-inositol separa tulen daripada dedak padi pada kepekatan <1000 mg/ml. Perencatan MCF-7 hanya dilihat pada kepekatan lebih daripada 30 mg/ml dengan lebih daripada 40% sel terencat. Ini menunjukkan bahawa fosfat myo-inositol daripada dedak padi separa tulen terdegradasi oleh fitat ASUIA279 terhadap sel kanser MCF-7 dimana ia menunjukkan keputusan positif terhadap perencatan tumbesaran sel kanser pada kepekatan tinggi.


Download data is not yet available.
How to Cite
Yusoff, N. M., Nuge, T., Zainan, N., Hashim, Y.-Y., Jamal, P., Meor Hussin, A. S., Farouk, A.-E., & Salleh, and H. (1). Preliminary Investigation of Myo-Inositol Phosphates Produced by ASUIA279 Phytase on MCF-7 Cancer Cells. IIUM Engineering Journal, 12(4).

Most read articles by the same author(s)