GROWTH REVIVAL OF GREY OYSTER (PLEUROTUS PULMONARIUS) POWDER CULTURE MUSHROOM FROM THE EFFECT OF SPRAY DRYING TEMPERATURE

Zarina Zakaria; Fatihah Aziz; Mohd Sharizan Md Sarip; Noor Hasyierah Mohd Salleh

doi:10.31436/iiumej.v25i1.2748

Authors

Zarina Zakaria Universiti Malaysia Perlis https://orcid.org/0009-0005-8544-6865
Fatihah Aziz Universiti Malaysia Perlis
Mohd Sharizan Md Sarip Universiti Malaysia Perlis https://orcid.org/0000-0001-6517-048X
Noor Hasyierah Mohd Salleh Universiti Malaysia Perlis https://orcid.org/0000-0001-8286-1362

DOI:

https://doi.org/10.31436/iiumej.v25i1.2748

Keywords:

spray drying; mushroom powder culture; grey oyster mushroom; growth revival

Abstract

In mushroom cultivation, spawn is among the most important factors that ensure a successful production. Considering several drawbacks from both solid and liquid spawn in shelf life and preservation matters, it is suggested that the dry powder form of spawn is a good potential to explore. A study of powder culture formation using a spray dryer involved various inlet temperatures of 80^oC, 90^oC, 100^oC, 110^oC, 120^oC and 130 ^oC. The yield % of the dried powder culture was measured and it was found that 130 ^oC yielded the highest percentage of 50.33%. The lowest temperature yielded the lowest percentage. On the other hand, the lowest temperature of 80 ^oC revived the highest mycelium dry weight at 1.68 g which was obtained on the 10^th day of the incubation periods. The revival ability was decreased with the increase of temperature. The study proved that the powder culture of P. pulmonarius was able to perform and revive whereby it holds a potential to be preserved over a longer period, which is beneficial for the mushroom cultivator.

ABSTRAK: Dalam penanaman cendawan, benih yang baik adalah antara faktor penting yang memastikan penghasilan tinggi. Terdapat beberapa kelemahan daripada benih pepejal dan cecair dalam memastikan jangka hayat berpanjangan serta memastikan benih dalam keadaan berkualiti sepanjang masa. Oleh itu, penghasilan benih cendawan dalam bentuk serbuk kering sangat berpotensi untuk diterokai. Kajian mengenai penghasilan kultur serbuk menggunakan semburan kering telah melibatkan pelbagai suhu salur masuk seperti 80^oC, 90^oC, 100^oC, 110^oC, 120^oC dan 130 ^oC. Peratus penghasilan kultur serbuk kering telah diukur dan didapati pada suhu 130^oC menghasilkan peratusan tertinggi iaitu sebanyak 50.33%, manakala suhu terendah menghasilkan peratusan terendah. Sebaliknya, suhu terendah pada 80^oC menumbuhkan semula berat kering miselium dengan bacaan tertinggi iaitu sebanyak 1.68 g diperoleh pada hari ke-10 tempoh inkubasi. Keupayaan menumbuh berkurangan dengan peningkatan suhu. Kajian membuktikan bahawa kultur serbuk P. pulmonarius mampu berfungsi dan tumbuh semula di mana ianya berpotensi disimpan dalam tempoh lama, ini berfaedah kepada penanaman cendawan.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Carrasco J, Zied DC, Navarro MJ, Gea FJ, Pardo-Giménez A. (2021) Commercial Cultivation Techniques of Mushrooms. In Advances in Macrofungi. 1st Edition. Edited by Kandikere RS, Sunil KD, Boca Raton, CRC Press; pp. 11-40. DOI: https://doi.org/10.1201/9781003096818-3

Kerketta A, Pandey NK, Singh HK. (2017) Evaluation of Different Grains for Spawn Development of Milky Mushroom (Calocybe indica, P&C). Research Journal of Agricultural Sciences, 8(1): 266-268.

Kumar V, Sahai V, Bisaria VS. (2012) Production of amylase and chlamydospores by Piriformospora indica, a root endophytic fungus. Biocatalysis and Agricultural Biotechnology, 1(2): 124-128. https://doi.org/10.1016/j.bcab.2012.02.002 DOI: https://doi.org/10.1016/j.bcab.2012.02.002

Braga ABAC, Costa CJM, Pomella AWV, Ribeiro E J, Santos LD, Zotarelli MF. (2019) Evaluation of lethality temperature and use of different wall materials in the microencapsulation process of Trichoderma asperellum conidias by spray drying. Powder Technology, 347: 199-206. https://doi.org/10.1016/j.powtec.2019.02.037 DOI: https://doi.org/10.1016/j.powtec.2019.02.037

Go WZ, H’ng PS, Wong MY, Chin KL, Ujang S, Noran AS. (2019) Evaluation of Trichoderma asperellum as a potential biocontrol agent against Rigidoporus microporus Hevea brasiliensis. Archives of Phytopathology and Plant Protection, 52(7-8): 639-666. https://doi.org/10.1080/03235408.2019.1587821 DOI: https://doi.org/10.1080/03235408.2019.1587821

Marcellin ML, François ME, Valteri VA, Endali EM-J, Begoude BAD. (2018) In vitro study of the compatibility of six fungicides with two strains of Trichoderma asperellum, biocontrol agents used against cacao black pod disease in Cameroon. International Journal of Innovation and Applied Studies, 24(4): 1834-1848.

http://www.ijias.issr-journals.org/abstract.php?article=IJIAS-18-184-01

Chávez BE, Ledeboer AM. (2007) Drying of probiotics: Optimization of formulation and process to enhance storage survival. Drying Technology, 25(7-8): 1193-1201. https://doi.org/10.1080/07373930701438576 DOI: https://doi.org/10.1080/07373930701438576

Silva J, Carvalho AS, Teixeira P, Gibbs PA. (2002) Bacteriocin production by spray-dried lactic acid bacteria. Letters in Applied Microbiology, 34(2): 77-81. https://doi.org/10.1046/J.1472-765X.2002.01055.X DOI: https://doi.org/10.1046/j.1472-765x.2002.01055.x

Silva J, Carvalho AS, Ferreira R, Vitorino R, Amado F, Domingues P, Teixeira P, Gibbs PA. (2005) Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying. Journal of Applied Microbiology, 98(3): 775-782. https://doi.org/10.1111/J.1365-2672.2004.02516.X DOI: https://doi.org/10.1111/j.1365-2672.2004.02516.x

Ishak AA, Zulkepli FRA, Hayin NFM, Md Zain NH, Sapak Z. (2021) Effect of high inlet temperature of spray dryer on viability of microencapsulated Trichoderma asperellum conidia. IOP Conference Series: Earth and Environmental Science, 757(1): 012023. https://doi.org/10.1088/1755-1315/757/1/012023 DOI: https://doi.org/10.1088/1755-1315/757/1/012023

Rosmiza M., Davies W., Aznie R. C., Jabil M., Mazdi M. (2016). Prospects for Increasing Commercial Mushroom Production in Malaysia: Challenges and Opportunities. Mediterranean Journal of Social Sciences, 7(1): 406-415. DOI: https://doi.org/10.5901/mjss.2016.v7n1s1p406

Zakaria Z, Islam MT, Hamidin N, Azlan MMI, Ishak BM. (2016) Characteristics of Indoor Mushroom Cultivation of Grey Oyster (Pleurotus pulmonarius) by Different Stages of Humidifying Treatment. World Applied Sciences Journal, 34(8): 1066-1075. https://doi.org/10.5829/idosi.wasj.2016.34.8.165

Chong SF, Zakaria Z, Low JZ. (2019) Investigation of chlamydospores and mycelium liquid spawn storage period of grey oyster mushroom (Pleurotus pulmonarius) and effects on growth rate. International Journal of Advances in Science Engineering and Technology, Sppl. 1: 2321-8991

Jin X, Custis D. (2011) Microencapsulating aerial conidia of Trichoderma harzianum through spray drying at elevated temperatures. Biological Control, 56 (2011) 202–208 DOI: https://doi.org/10.1016/j.biocontrol.2010.11.008

Tran TTA, Nguyen HVH. (2018) Effects of Spray-Drying Temperatures and Carriers on Physical and Antioxidant Properties of Lemongrass Leaf Extract Powder. Beverages, 4(4): 84. https://doi.org/10.3390/beverages4040084 DOI: https://doi.org/10.3390/beverages4040084

Reineccius GA. (2004) The spray drying of food flavors. Drying Technology, 22(6): 1289-1324. https://doi.org/10.1081/DRT-120038731 DOI: https://doi.org/10.1081/DRT-120038731

Poirier T, Cheng R, Cardoso R, Gutie?rrez Y, Mari E (2016) Characterisation and reduction of 'wall sticking' in spray-drying clay suspensions in QUALICER 2006: IX World Congress on Ceramic Tile Quality; Castellón (Spain).

Nijdam JJ, Langrish TAG. (2006) The effect of surface composition on the functional properties of milk powders. Journal of Food Engineering, 77(4): 919-925. https://doi.org/10.1016/J.JFOODENG.2005.08.020 DOI: https://doi.org/10.1016/j.jfoodeng.2005.08.020

Mansor MR, Md Sarip MS, Nik Daud NMA, Saidi SA, Mohd Nawi MAH, Jamlos MA. (2022) Preparation of Rice Bran Protein (RBP) Powder Using Spray Drying Method at the Optimal Condition and Its Protein Quality. Processes. 10(10): 2026. https://doi.org/10.3390/pr10102026 DOI: https://doi.org/10.3390/pr10102026

Abdullah N, Ismail R, Johari NMK, Annuar MSM. (2013) Production of liquid spawn of an edible grey oyster mushroom, Pleurotus pulmonarius (Fr.) Quél by submerged fermentation and sporophore yield on rubber wood sawdust. Scientia Horticulturae, 161: 65-69. https://doi.org/10.1016/j.scienta.2013.06.026 DOI: https://doi.org/10.1016/j.scienta.2013.06.026

Betoret E, Betoret N, Calabuig-Jiménez L, Barrera C, Dalla Rosa M. (2020) Effect of drying process, encapsulation, and storage on the survival rates and gastrointestinal resistance of L. salivarius spp. salivarius included into a fruit matrix. Microorganisms, 8(5): 654. https://doi.org/10.3390/MicroorganismS8050654 DOI: https://doi.org/10.3390/microorganisms8050654