CYTOTOXICITY EFFECT OF IONIC LIQUID-GRAVIOLA FRUIT (ANNONA MURICATA) EXTRACT TO HUMAN COLON CANCER (HT29) CELL LINES

Authors

  • Djabir Daddiouaissa International Islamic University Malaysia https://orcid.org/0000-0002-9999-162X
  • Azura Amid International Institute for Halal Research and Training (INHART)
  • Nassereldeen Ahmed Kabbashi Biotechnology Engineering Department https://orcid.org/0000-0002-1524-5065
  • Ahmed Adam Mohammed Elnour International Institute for Halal Research and Training (INHART) https://orcid.org/0000-0002-1924-094X
  • Mohamad Adika Khairy Bin Mohd Shaifudin Epandy Adikafirdaus Resources

DOI:

https://doi.org/10.31436/iiumej.v22i2.1687

Keywords:

Colon cancer, Flow cytometry, Graviola (Annona muricata), GC-TOFMS, Ionic liquids

Abstract

The present study aimed to investigate the anti-proliferative effect of the ionic liquid-Graviola fruit (IL-GFE) extract on colon adenocarcinoma (HT29) cell lines and their kinetics behaviour to assess the Graviola fruit potential as a therapeutic alternative in cancer treatment. The phytoconstituents content of IL-GFE was identified using GC-TOFMS apparatus and measured its cytotoxicity on HT29 by tetrazolium bromide. Then the cytokinetic behaviour of the treated HT29 cells with IL-GFE was illustrated using the cells' growth curve. Besides, the cell cycle phase perturbation for the treated HT29 was applied using a flow cytometry technique. Qualitative identification of phytoconstituents of IL-GFE showed that Graviola fruit contains acetogenins, alkaloids, flavonoids, tannins and saponins compounds. IL-GF extract displayed a cytotoxicity effect on HT29 cells with the IC50 value of 10.56 µg/mL, while Taxol showed an IC50 value of 1.22 µg/mL. IL-GFE also decreased the cell generation number from 3.93 to 2.96 generations compared to Taxol-treated cells 2.01 generations. The microscope observation of the HT29 cells treated with the crude IL-GFE displayed loss of density and cell detachment. The extract's growth inhibition was related to the cell cycle arrest at the G0/G1 phase. IL-GFE inhibited colon adenocarcinoma HT29 cells' proliferation and affected their kinetic behaviour by lowering cell viability, inducing apoptosis, and arresting the cell cycle at the G0/G1 phase.

ABSTRAK: Kajian ini bertujuan untuk mengkaji kesan anti-proliferatif ekstrak buah-ion Graviola (IL-GFE) pada garis sel adenokarsinoma kolon (HT29) dan tingkah laku kinetik mereka untuk menilai potensi buah Graviola sebagai alternatif terapi untuk barah rawatan. Kandungan fitokonstituen IL-GFE dikenal pasti menggunakan alat GC-TOFMS dan mengukur sitotoksisitasnya pada HT29 oleh tetrazolium bromida. Kemudian tingkah laku sitokinetik sel HT29 yang dirawat dengan IL-GFE digambarkan menggunakan keluk pertumbuhan sel. Selain itu, gangguan fasa kitaran sel untuk HT29 yang dirawat diaplikasikan menggunakan teknik sitometri aliran. Pengenalpastian kualitatif fitokonstituen IL-GFE menunjukkan bahawa buah Graviola mengandungi asetogenin, alkaloid, flavonoid, tanin dan sebatian saponin. Ekstrak IL-GF memperlihatkan kesan sitotoksisiti pada sel HT29 dengan nilai IC50 10.56 µg/mL, sementara Taxol menunjukkan nilai IC50 1.22 µg/mL. IL-GFE juga menurunkan jumlah penjanaan sel dari 3.93 hingga 2.96 generasi berbanding sel yang dirawat Taxol 2.01 generasi. Pemerhatian mikroskop sel HT29 yang dirawat dengan IL-GFE kasar menunjukkan kehilangan ketumpatan dan detasmen sel. Perencatan pertumbuhan ekstrak berkaitan dengan penangkapan kitaran sel pada fasa G0/G1. IL-GFE menghalang percambahan sel HT29 adenokarsinoma kolon dan mempengaruhi tingkah laku kinetik mereka dengan menurunkan daya maju sel, mendorong apoptosis, dan menghentikan kitaran sel pada fasa G0/G1.

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References

Patel MS, Patel JK. (2016) A review on a miracle fruits of Annona muricata. Journal of Pharmacognosy and Phytochemistry, 5(1): 137-148.

Ioannis P, Anastasis S, Andreas Y. (2015) Graviola: A systematic review on its anti-cancer properties. Am. J. Cancer Prev, 3(6): 128-131. https://doi.org/10.12691/ajcp-3-6-5 DOI: https://doi.org/10.12691/ajcp-3-4-3

Yajid AI, Ab Rahman HS, Wong MPK, Zain WZW. (2018) Potential benefits of Annona muricata in combating cancer: A Review. The Malaysian Journal of Medical Sciences, 25(1): 5-15. https://dx.doi.org/10.21315%2Fmjms2018.25.1.2 DOI: https://doi.org/10.21315/mjms2018.25.1.2

Badrie N, Schauss AG. (2010) Soursop (Annona muricata L.): composition, nutritional value, medicinal uses, and toxicology. Bioactive Foods in Promoting Health, Elsevier, pp. 621-643. https://doi.org/10.1016/B978-0-12-374628-3.00039-6 DOI: https://doi.org/10.1016/B978-0-12-374628-3.00039-6

Coe FG. (2008) Rama midwifery in eastern Nicaragua. Journal of Ethnopharmacology, 117(1): 136-157. https://doi.org/10.1016/j.jep.2008.01.027 DOI: https://doi.org/10.1016/j.jep.2008.01.027

Samuel AJSJ, Kalusalingam A, Chellappan DK, Gopinath R, Radhamani S, Husain HA, Muruganandham V, Promwichit P. (2010) Ethnomedical survey of plants used by the Orang Asli in Kampung Bawong, Perak, West Malaysia. Journal of Ethnobiology and Ethnomedicine, 6(1): 1-6. https://doi.org/10.1186/1746-4269-6-5 DOI: https://doi.org/10.1186/1746-4269-6-5

Tisott G, Molin D, Colet C. (2013) The use of medicinal plants and herbal medicines for patients in chemotherapy in an oncology center of Iju?/RS. O Mundo da Saude, 39(3): 287-298. DOI: https://doi.org/10.15343/0104-7809.20153903287298

George VC, Kumar D, Rajkumar V, Suresh P, Kumar RA. (2012) Quantitative assessment of the relative antineoplastic potential of the n-butanolic leaf extract of Annona muricata Linn. in normal and immortalised human cell lines. Asian Pacific Journal of Cancer Prevention, 13(2): 699-704. https://doi.org/10.7314/APJCP.2012.13.2.699 DOI: https://doi.org/10.7314/APJCP.2012.13.2.699

Monigatti M, Bussmann RW, Weckerle CS. (2013) Medicinal plant use in two Andean communities located at different altitudes in the Bolívar Province, Peru. Journal of Ethnopharmacology, 145(2): 450-464. https://doi.org/10.1016/j.jep.2012.10.066 DOI: https://doi.org/10.1016/j.jep.2012.10.066

Betancur-Galvis LA, Saez J, Granados H, Salazar A, Ossa J. (1999) Antitumor and antiviral activity of Colombian medicinal plant extracts. Memórias do Instituto Oswaldo Cruz, 94(4): 531-535. http://dx.doi.org/10.1590/S0074-02761999000400019 DOI: https://doi.org/10.1590/S0074-02761999000400019

Dai Y, Hogan S, Schmelz EM, Ju YH, Canning C, Zhou K. (2011) Selective growth inhibition of human breast cancer cells by graviola fruit extract in vitro and in vivo involving downregulation of EGFR expression. Nutrition and Cancer, 63(5): 795-801. https://doi.org/10.1080/01635581.2011.563027 DOI: https://doi.org/10.1080/01635581.2011.563027

Gavamukulya Y, Abou-Elella F, Wamunyokoli F, AEl-Shemy H. (2014) Phytochemical screening, anti-oxidant activity and in vitro anti-cancer potential of ethanolic and water leaves extracts of Annona muricata (Graviola). Asian Pacific Journal of Tropical Medicine, 7(13): S355-S363. https://doi.org/10.1016/S1995-7645(14)60258-3 DOI: https://doi.org/10.1016/S1995-7645(14)60258-3

Daddiouaissa D, Amid A. (2018) Anti-cancer activity of acetogenins from Annona muricata fruit. International Medical Journal Malaysia, 17(3): 103-112. https://doi.org/10.31436/imjm.v17i3.236 DOI: https://doi.org/10.31436/imjm.v17i3.236

Sun S, Liu J, Sun X, Zhu W, Yang F, Felczak L, Dou QP, Zhou K. (2017) Novel Annonaceous acetogenins from Graviola (Annona muricata) fruits with strong anti-proliferative activity. Tetrahedron Letters, 58(19): 1895-1899. https://doi.org/10.1016/j.tetlet.2017.04.016 DOI: https://doi.org/10.1016/j.tetlet.2017.04.016

Daddiouaissa D, Amid A, Kabbashi NA, Fuad FA, Elnour AM, Epandy MA. (2019) Anti-proliferative activity of ionic liquid-Graviola fruit extract against human breast cancer (MCF-7) cell lines using flow cytometry techniques. Journal of Ethnopharmacology, 236: 466-473. https://doi.org/10.1016/j.jep.2019.03.003 DOI: https://doi.org/10.1016/j.jep.2019.03.003

Siegel RL, Miller KD, Jemal A. (2019) Cancer statistics, 2019. CA: A cancer journal for clinicians 69(1), 7-34. https://doi.org/10.3322/caac.21551 DOI: https://doi.org/10.3322/caac.21551

Griffin A, Butow P, Coates A, Childs A, Ellis P, Dunn S, Tattersall M. (1996) On the receiving end V: patient perceptions of the side effects of cancer chemotherapy in 1993. Annals of Oncology, 7(2): 189-195. https://doi.org/10.1093/oxfordjournals.annonc.a010548 DOI: https://doi.org/10.1093/oxfordjournals.annonc.a010548

Daddiouaissa D, Amid A, Syahida A, Elnour AM. (2020) Phytochemical analysis of ionic liquid-Graviola (Annona muricata) fruit extract and its acute toxicity on zebrafish early-life stages. Asia Pacific Journal of Molecular Biology and Biotechnology, 28(2): 113-124. https://doi.org/10.35118/apjmbb.2020.028.2.10 DOI: https://doi.org/10.35118/apjmbb.2020.028.2.10

Koel M, Kuhtinskaja M, Vaher M. (2020) Extraction of bioactive compounds from Catharanthus roseus and Vinca minor. Separation and Purification Technology, 252: 117438. https://doi.org/10.1016/j.seppur.2020.117438 DOI: https://doi.org/10.1016/j.seppur.2020.117438

Ventura SPM, e Silva FA, Quental MV, Mondal D, Freire MG, Coutinho JAP. (2017) Ionic-liquid-mediated extraction and separation processes for bioactive compounds: Past, present, and future trends. Chemical Reviews, 117(10): 6984-7052. https://doi.org/10.1021/acs.chemrev.6b00550 DOI: https://doi.org/10.1021/acs.chemrev.6b00550

Bogdanov MG. (2014) Ionic liquids as alternative solvents for extraction of natural products, alternative solvents for natural products extraction, Springer, pp 127-166. DOI: https://doi.org/10.1007/978-3-662-43628-8_7

Cláudio AFM, Marques CF, Boal-Palheiros I, Freire MG, Coutinho JA. (2014) Development of back-extraction and recyclability routes for ionic-liquid-based aqueous two-phase systems. Green Chemistry, 16(1): 259-268. https://doi.org/10.1039/C3GC41999A DOI: https://doi.org/10.1039/C3GC41999A

Wang T, Wang Q, Li P, Yang H. (2019) Temperature-responsive ionic liquids to set up a method for the simultaneous extraction and in situ preconcentration of hydrophilic and lipophilic compounds from medicinal plant matrices. Green Chemistry, 21(15): 4133-4142. https://doi.org/10.1039/C9GC00995G DOI: https://doi.org/10.1039/C9GC00995G

Fan Y, Li W, Zhang S, Sun S, Yang L. (2020) Vitamin B3-based protic ionic liquids as green solvents for the isolation of astilbin from rhizoma smilacis glabrae. Industrial Crops and Products, 152: 112563. https://doi.org/10.1016/j.indcrop.2020.112563 DOI: https://doi.org/10.1016/j.indcrop.2020.112563

Hoenig M. (2001) Preparation steps in environmental trace element analysis—facts and traps. Talanta, 54(6): 1021-1038. https://doi.org/10.1016/S0039-9140(01)00329-0 DOI: https://doi.org/10.1016/S0039-9140(01)00329-0

Gyamfi K, Sarfo D, Nyarko B, Akaho E, Serfor-Armah Y, Ampomah-Amoako E. (2011) Assessment of elemental content in the fruit of graviola plant, Annona muricata, from some selected communities in Ghana by instrumental neutron activation analysis. Elixir Food Sci, 41: 5671-5675.

Rahman NRHA, Idris A, Yunus NA, Mustaffa AA. (2019) Optimisation of ionic liquid-based microwave extraction of flavonoid and phenolic acid from Labisia pumila. AIP Conference Proceedings (Vol. 2124, No. 1, p. 020027), AIP Publishing LLC. https://doi.org/10.1063/1.5117087 DOI: https://doi.org/10.1063/1.5117087

Bhan M, Satija S, Garg C, Dureja H, Garg M. (2017) A novel approach towards green extraction for glycyrrhitinic acid by ionic liquid based microwave assisted extraction and optimisation through response surface methodology. Pharmacognosy Journal, 9(6): 866-872. https://doi.org/10.5530/pj.2017.6.136 DOI: https://doi.org/10.5530/pj.2017.6.136

Zhang Y, Liu Z, Li Y, Chi R. (2014) Optimisation of ionic liquid-based microwave-assisted extraction of isoflavones from Radix puerariae by response surface methodology. Separation and Purification Technology, 129: 71-79. https://doi.org/10.1016/j.seppur.2014.03.022 DOI: https://doi.org/10.1016/j.seppur.2014.03.022

Jang Z-H, Chung H-C, Ahn YG, Kwon Y-K, Kim J-S, Ryu J-H, Kim C-H, Hwang G-S. (2012) Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio). Molecular BioSystems, 8(7): 2001-2009. https://doi.org/10.1039/C2MB25073J DOI: https://doi.org/10.1039/c2mb25073j

Muhamad S, Jamilah B, Russly A, Faridah A. (2017) In vitro antibacterial activities and composition of Carica papaya cv. Sekaki/Hong Kong peel extracts. International Food Research Journal, 24(3): p976-984.

Liu N, Yang HL, Wang P, Lu YC, Yang YJ, Wang L, Lee SC. (2016) Functional proteomic analysis revels that the ethanol extract of Annona muricata L. induces liver cancer cell apoptosis through endoplasmic reticulum stress pathway. Journal of Ethnopharmacology, 189: 210-217. https://doi.org/10.1016/j.jep.2016.05.045 DOI: https://doi.org/10.1016/j.jep.2016.05.045

Chik WW, Amid A, Jamal P. (2010) Purification and cytotoxicity assay of tomato (Lycopersicon esculentum) leaves methanol extract as potential anti-cancer agent. Journal of Applied Sciences, 10(24): 3283-3288. https://dx.doi.org/10.3923/jas.2010.3283.3288 DOI: https://doi.org/10.3923/jas.2010.3283.3288

Fouz N, Amid A, Hashim Y. (2013) Cytokinetic study of MCF-7 cells treated with commercial and recombinant bromelain. Asian Pacific Journal of Cancer Prevention, 14(11): 6709-6714. https://doi.org/10.7314/APJCP.2013.14.11.6709 DOI: https://doi.org/10.7314/APJCP.2013.14.11.6709

Magadi VP, Ravi V, Arpitha A. (2015) Evaluation of cytotoxicity of aqueous extract of Graviola leaves on squamous cell carcinoma cell-25 cell lines by 3-(4, 5-dimethylthiazol-2-Yl)-2, 5-diphenyltetrazolium bromide assay and determination of percentage of cell inhibition at G2M phase of cell cycle by flow cytometry: An in vitro study. Contemporary Clinical Dentistry, 6(4): 529–533. https://dx.doi.org/10.4103%2F0976-237X.169863 DOI: https://doi.org/10.4103/0976-237X.169863

Agu KC, Okolie NP, Falodun A, Engel-Lutz N. (2018) In vitro anti-cancer assessments of Annona muricata fractions and in vitro anti-oxidant profile of fractions and isolated acetogenin (15-acetyl guanacone). Journal of Cancer Research and Practice, 5(2): 53-66. https://doi.org/10.1016/j.jcrpr.2017.12.001 DOI: https://doi.org/10.1016/j.jcrpr.2017.12.001

Suhendar U. (2018) Geographical Effect on the Cytotoxic Activity of Annona muricata L. Leaves Extract Against MCF7 Cancer Cell. Fitofarmaka, 8(2): 12-19. DOI: https://doi.org/10.33751/jf.v8i2.1065

Mehrara E, Forssell-Aronsson E, Ahlman H, Bernhardt P. (2007) Specific growth rate versus doubling time for quantitative characterisation of tumor growth rate. Cancer Research, 67(8): 3970-3975. https://doi.org/10.1158/0008-5472.CAN-06-3822 DOI: https://doi.org/10.1158/0008-5472.CAN-06-3822

Wang H, Oo Khor T, Shu L, Su Z-Y, Fuentes F, Lee J-H, Tony Kong A-N. (2012) Plants vs. cancer: a review on natural phytochemicals in preventing and treating cancers and their druggability. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents), 12(10): 1281-1305. https://doi.org/10.2174/187152012803833026 DOI: https://doi.org/10.2174/187152012803833026

Boik J. (2001) Natural compounds in cancer therapy (Vol. 851). Oregon Medical Press, 1st ed.

Coria-Tellez AV, Montalvo-Gónzalez E, Yahia EM, Obledo-Vázquez EN. (2018) Annona muricata: A comprehensive review on its traditional medicinal uses, phytochemicals, pharmacological activities, mechanisms of action and toxicity. Arabian Journal of Chemistry, 11(5): 662-691. https://doi.org/10.1016/j.arabjc.2016.01.004 DOI: https://doi.org/10.1016/j.arabjc.2016.01.004

Nazeer AA, Veeraiyan S, Vijaykumar SD. (2017) Anti-cancer potency and sustained release of phytosomal diallyl disulfide containing methanolic Allium sativum extract against breast cancer. International Research Journal of Pharmacy, 8(8): 34-40. https://doi.org/10.7897/2230-8407.088141 DOI: https://doi.org/10.7897/2230-8407.088141

Nazarov A, Nosova YN, Mikhalev O, Kovaleva O, Dyson P, Milaeva E. (2016) Anti-proliferative activity of ruthenium and osmium clusters with phosphine ligands. Russian Chemical Bulletin, 65(2): 546-549. https://doi.org/10.1007/s11172-016-1335-x DOI: https://doi.org/10.1007/s11172-016-1335-x

Nowak-Sliwinska P, Zava O, Van Beijnum JR, Groessl M, Chisholm DM, Ahmadi Z, McIndoe JS, Griffioen AW. (2013) Synthesis and characterisation of a new class of anti-angiogenic agents based on ruthenium clusters. Scientific Reports, 3: 1485. https://doi.org/10.1038/srep01485 DOI: https://doi.org/10.1038/srep01485

Belayachi L, Aceves-Luquero C, Merghoub N, de Mattos SF, Amzazi S, Villalonga P, Bakri Y. (2017) Induction of cell cycle arrest and apoptosis by Ormenis eriolepis a Morrocan endemic plant in various human cancer cell lines. African Journal of Traditional, Complementary and Alternative Medicines, 14(2): 356-373. https://doi.org/10.21010/ajtcam.v14i2.37 DOI: https://doi.org/10.21010/ajtcam.v14i2.37

Cheng W, Liang C, Xu L, Liu G, Gao N, Tao W, Luo L, Zuo Y, Wang X, Zhang X. (2017) TPGS?functionalized polydopamine?modified mesoporous silica as drug nanocarriers for enhanced lung cancer chemotherapy against multidrug resistance. Small, 13(29): 1-12. https://doi.org/10.1002/smll.201700623 DOI: https://doi.org/10.1002/smll.201700623

Sui L, Dong Y, Watanabe Y, Yamaguchi F, Hatano N, Tsukamoto I, Izumori K, Tokuda M. (2005) The inhibitory effect and possible mechanisms of D-allose on cancer cell proliferation. International Journal of Oncology, 27(4): 907-912. https://doi.org/10.3892/ijo.27.4.907 DOI: https://doi.org/10.3892/ijo.27.4.907

Ban JO, Hwang IG, Kim TM, Hwang BY, Lee US, Jeong H-S, Yoon YW, Kim DJ, Hong JT. (2007) Anti-proliferate and pro-apoptotic effects of 2, 3-dihydro-3, 5-dihydroxy-6-methyl-4H-pyranone through inactivation of NF-?B in human colon cancer cells. Archives of Pharmacal Research, 30(11): 1455. https://doi.org/10.1007/BF02977371 DOI: https://doi.org/10.1007/BF02977371

Kameue C, Tsukahara T, Ushida K. (2006) Alteration of gene expression in the colon of colorectal cancer model rat by dietary sodium gluconate. Bioscience, Biotechnology, and Biochemistry, 70(3): 606-614. https://doi.org/10.1271/bbb.70.606 DOI: https://doi.org/10.1271/bbb.70.606

Consolacion Y, Geneveve S, Ming-Jaw D, Chien-Chang S. (2012) Acetogenins from Annona muricata. Pharmacognosy Journal, 4(32): 32-37. https://doi.org/10.5530/pj.2012.32.7 DOI: https://doi.org/10.5530/pj.2012.32.7

Sun S, Liu J, Kadouh H, Sun X, Zhou K. (2014) Three new anti-proliferative Annonaceous acetogenins with mono-tetrahydrofuran ring from graviola fruit (Annona muricata). Bioorganic & Medicinal Chemistry Letters, 24(12): 2773-2776. https://doi.org/10.1016/j.bmcl.2014.03.099 DOI: https://doi.org/10.1016/j.bmcl.2014.03.099

Sun S, Liu J, Zhou N, Zhu W, Dou QP, Zhou K. (2016) Isolation of three new annonaceous acetogenins from Graviola fruit (Annona muricata) and their anti-proliferation on human prostate cancer cell PC-3. Bioorganic & Medicinal Chemistry Letters, 26(17): 4382-4385. https://doi.org/10.1016/j.bmcl.2015.06.038 DOI: https://doi.org/10.1016/j.bmcl.2015.06.038

Pieme CA, Kumar SG, Dongmo MS, Moukette BM, Boyoum FF, Ngogang JY, Saxena AK. (2014) Anti-proliferative activity and induction of apoptosis by Annona muricata (Annonaceae) extract on human cancer cells. BMC Complementary and Alternative Medicine, 14(1): 516. https://doi.org/10.1186/1472-6882-14-516 DOI: https://doi.org/10.1186/1472-6882-14-516

Daddiouaissa D, Amid A, Sani MSA, Elnour AA. (2021) Evaluation of metabolomics behavior of human colon cancer HT29 cell lines treated with ionic liquid graviola fruit pulp extract. Journal of Ethnopharmacology, 270: 113813. https://doi.org/10.1016/j.jep.2021.113813 DOI: https://doi.org/10.1016/j.jep.2021.113813

O'Reilly MS, Boehm T, Shing Y, Fukai N, Vasios G, Lane WS, Flynn E, Birkhead JR, Olsen BR, Folkman J. (1997) Endostatin: an endogenous inhibitor of angiogenesis and tumor growth. Cell, 88(2): 277-285. https://doi.org/10.1016/S0092-8674(00)81848-6 DOI: https://doi.org/10.1016/S0092-8674(00)81848-6

Mantena SK, Sharma SD, Katiyar SK. (2006) Berberine, a natural product, induces G1-phase cell cycle arrest and caspase-3-dependent apoptosis in human prostate carcinoma cells. Molecular Cancer Therapeutics, 5(2): 296-308. https://doi.org/10.1158/1535-7163.MCT-05-0448 DOI: https://doi.org/10.1158/1535-7163.MCT-05-0448

Moghadamtousi SZ, Karimian H, Rouhollahi E, Paydar M, Fadaeinasab M, Kadir HA. (2014) Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondria-mediated pathway in human HCT-116 and HT-29 colon cancer cells. Journal of Ethnopharmacology, 156: 277-289. https://doi.org/10.1016/j.jep.2014.08.011 DOI: https://doi.org/10.1016/j.jep.2014.08.011

Chamcheu J, Rady I, Chamcheu R-C, Siddique A, Bloch M, Banang Mbeumi S, Babatunde A, Uddin M, Noubissi F, Jurutka P. (2018) Graviola (Annona muricata) exerts anti-proliferative, anti-clonogenic and pro-apoptotic effects in human non-melanoma skin cancer UW-BCC1 and A431 cells in vitro: Involvement of hedgehog signaling. International Journal of Molecular Sciences, 19(6): 1791. https://doi.org/10.3390/ijms19061791 DOI: https://doi.org/10.3390/ijms19061791

Torres MP, Rachagani S, Purohit V, Pandey P, Joshi S, Moore ED, Johansson SL, Singh PK, Ganti AK, Batra SK. (2012) Graviola: A novel promising natural-derived drug that inhibits tumorigenicity and metastasis of pancreatic cancer cells in vitro and in vivo through altering cell metabolism. Cancer Letters, 323(1): 29-40. https://doi.org/10.1016/j.canlet.2012.03.031 DOI: https://doi.org/10.1016/j.canlet.2012.03.031

Gajalakshmi S, Vijayalakshmi S, Devi Rajeswari V. (2012) Phytochemical and pharmacological properties of Annona muricata: A review. International Journal of Pharmacy and Pharmaceutical Sciences, 4(2): 3-6.

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2021-07-04

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Daddiouaissa, D., Amid, A., AHMED KABBASHI, N. ., ADAM MOHAMMED ELNOUR, A. ., & ADIKA KHAIRY BIN MOHD SHAIFUDIN EPANDY, M. . (2021). CYTOTOXICITY EFFECT OF IONIC LIQUID-GRAVIOLA FRUIT (ANNONA MURICATA) EXTRACT TO HUMAN COLON CANCER (HT29) CELL LINES. IIUM Engineering Journal, 22(2), 50–66. https://doi.org/10.31436/iiumej.v22i2.1687

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Chemical and Biotechnology Engineering