Some Biological Activities of Malaysian Leech Saliva Extract

  • Abdualrahman M. Abdualkader IIUM
  • Ahmed Merzouk A. Merzouk
  • Abbas Mohammed Ghawi A. M. Ghawi
  • and Mohammed Alaama IIUM
Keywords: leech, anticoagulant, thrombin time, antibacterial activity.

Abstract

Leeches were fed on the phagostimulatory solution through parafilm membrane. The satiated leeches were forced to regurgitate the solution by soaking them in an ice-container. The anticoagulant activity was ascertained using thrombin time assay (TT). The result revealed that the saliva concentration which increases TT by 100% (IC100) is 43.205µg/ml plasma. The antimicrobial activity of the saliva was tested against several bacterial spp. (E.coli, P.aeruginosa, B.cereus, Sal.typhi and S.aureus)  and fungi spp. (C.albicans and C.neoformans). It was found that saliva has an inhibition activity against Sal.typhi (minimal inhibitory concentration MIC 78.253µg/ml), S.aureus (MIC 78.253µg/ml) and E.coli (MIC 121.256µg/ml).

ABSTRAK: Pacat-pacat diberi makan larutan phagostimulatory menerusi membran parafilem. Pacat-pacat yang kekenyangan itu dipaksa memuntahkan larutan tersebut dengan direndam di dalam bekas berisi ais. Aktiviti antigumpal ditentukan menggunakan cerakin masa trombin (TT). Keputusan menunjukkan kepekatan air liur pacat menyebabkan pertambahan TT sebanyak 100% (IC100) iaitu 43.205µg/ml plasma. Aktiviti antimikrob air liur telah diuji dengan pelbagai jenis bakteria (E.coli, P.aeruginosa, B.cereus, Sal.typhi dan S.aureus) dan pelbagai jenis kulat (C.albicans and C.neoformans). Didapati air liur menghasilkan aktiviti perencatan terhadap Sal.typhi (kepekatan perencat minima (Minimal inhibitory concentration - MIC) 78.253µg/ml), S.aureus (MIC 78.253µg/ml) dan E.coli (MIC 121.256µg/ml).

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Author Biographies

Abdualrahman M. Abdualkader, IIUM
Master candidate,

Pharmaceutical Chemistry Department,

Kulliyyah of Pharmacy, International Islamic University Malaysia

Ahmed Merzouk, A. Merzouk

Asoc. Prof.

Pharmaceutical Chemistry department

Kulliyyah of Pharmacy, IIUM

 

Abbas Mohammed Ghawi, A. M. Ghawi

Prof. Dr.

Basic medical science department

Kulliyyah of Pharmacy, IIUM

and Mohammed Alaama, IIUM
Master candidate,

Pharmaceutical Chemistry Department,

Kulliyyah of Pharmacy, International Islamic University Malaysia

Published
2011-12-29
How to Cite
M. Abdualkader, A., Merzouk, A., Ghawi, A. M., & Alaama, and M. (2011). Some Biological Activities of Malaysian Leech Saliva Extract. IIUM Engineering Journal, 12(4). https://doi.org/10.31436/iiumej.v12i4.156