BIOELECTROCHEMICAL BEHAVIOR OF WILD TYPE BACILLUS CEREUS IN DUAL CHAMBER MICROBIAL FUEL CELL

M Amirul Islam

Abstract


Microbial fuel cell (MFC) is a bioelectrochemical system that uses bacteria as biocatalyst to oxidize organic substrates as well as release electrons, which can be harvested in an external circuit to produce electrical energy. In this study, a proteolytic biocatalyst Bacillus cereus (B. cereus) has been employed for the first time in a microbial fuel cell (MFC). The wild type pure culture was isolated from municipal wastewater and identified using Biolog Gen III analysis. The MFCs were fueled with palm oil mill effluent (POME) and attained the maximum power density of about 3.88 W/m3. The electrochemical behavior of MFC operated by B. cereus was evaluated using polarization curve, electrochemical impedance spectroscopy (EIS) and cyclic voltammetery (CV) analysis. B. Cereus excreated electron shuttling compound which significantly reduced the anode charge transfer resistance (52.95%). The FESEM result shows that B. Cereus has the capability of effective biofilm formation. These results revealed the electrocatalytic potentiality of B. cereus and which makes it a promising candidate to be used in MFCs. Therfore, this biocatlyst can be used to generate electricity through the wastewater valorization.

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