Assessing the feasibility of micro-computed tomography in comparing mineral densities and volume values of enamel and dentine in permanent premolars which were extracted teeth for orthodontic and periodontal treatment

Authors

  • Semsettin Yildiz F?rat University, Department of Pediatric Dentistry / Elaz?g, Turkey
  • Izzet Yavuz Dicle University, Department of Pediatric Dentistry / Diyarbakir, Turkey
  • Hakan Kamalak Sutcu ?mam University, Department of Restorative Dentistry / Kahramanmaras, Turkey
  • Zeki Akkus Dicle University, Department of Biostatistics / Diyarbakir, Turkey

DOI:

https://doi.org/10.31436/ijohs.v3i2.138

Keywords:

dentin, enamel, micro-computerized tomography, mineral density, volume

Abstract

The objective of our study was to show that the volumes of enamel-dentin tissues and mineral tissue densities of the teeth of young and adult individuals extracted for orthodontic and periodontal purposes could be measured using micro-computerized tomography. Non-decayed teeth extracted due to orthodontic and periodontal reasons were used. The teeth were scanned using a micro-CT (Skyscan 1172, Bruker, Belgium) device. The image data of the samples scanned with micro-CT were used in computer settings through the CTAn program for the calculation of the volumes of enamel and dentin tissues and their mineral densities. Comparisons between groups showed that there is no statistically significant difference between occlusal, middle, or apical zone mineral density values of the enamel and dentin tissues of the teeth in group 1 and group 2 (p>0,05). In addition, no statistically significant difference was detected between the mineral density values of average enamel and dentin tissues. Comparison between groups themselves showed a statistically significant difference between percentage ratios of enamel, dentin, and pulp volume compared to crown volume (p<0.05). We believe that the micro-CT technique is an imaging method that can perform accurate and sensitive measurements meant of volume changes observed in tooth tissues with time. In addition, we concluded that with micro-CT, the densities in enamel and dentin tissues in study groups could be measured reliably.

References

Aboshi, H., Takahashi, T., Komuro, T. (2010). Age estimation using microfocus X-ray computed tomography of lower premolars. Forensic Science International, 200, 35–40.

Agematsu, H., Someda, H., Hashimoto, M., Matsunaga, S, Abe, S., Kim, H-J., et al. (2010). Three-dimensional observation of a decrease in pulp cavity volume using micro-CT: age-related change. The Bulletin of Tokyo Dental College, 51(1), 1–6.

Bath-Balogh, M, Fehrenbach, M. (2006). Illustrated Dental Embryology, Histology, and Anatomy 2nd Ed. St. Louis: Elsevier Inc, pp. 61-91, 179-200.

Clementino-Luedemann, T.N.R., Kunzelmann, K-H. (2006). Mineral concentration of natural human teeth by a commercial micro-CT. Dental Materials Journal, 25(1), 113–119.

Davis, G.R., Wong, F.S.L. (1996) X-ray microtomography of bones and teeth. Physiological Measurement, 17(3), 121–146.

Djomehri, S.I., Candell, S., Case, T., Browning, A., Marshall, G.W., Yun, W., et al. (2015). Mineral density volume gradients in normal and diseased human tissues. Aikawa E, editor. PLoS One, 10(4).

Dogan, M., Callea, M., Kusdhany, L., Aras, A., Maharani, D., Mandasari, M., et al. (2018). The evaluation of root fracture with Cone Beam Computed Tomography (CBCT): an epidemiological study. Journal of Clinical and Experimental Dentistry, 10(1).

Dowker, S.E.P., Elliott, J.C., Davis, G.R., Wilson, R.M., Cloetens, P. (2006). Three-dimensional study of human dental fissure enamel by synchrotron X-ray microtomography. European Journal of Oral Sciences, 114 Suppl, 1, 353-359.

Farah, R.A., Swain, M.V., Drummond, B.K., Cook, R., Atieh, M. (2010). Mineral density of hypomineralised enamel. Journal of Dentistry, 38(1), 50–58.

Gantt, D.G., Kappleman, J., Ketcham, R.A., Alder, M.E., Deahl, T.H. (2006). Three-dimensional reconstruction of enamel thickness and volume in humans and hominoids. European Journal of Oral Sciences, 114, 360–4.

Hayashi-Sakai, S., Sakamoto, M., Hayashi, T., Kondo, T., Sugita, K., Sakai, J., et al. (2018). Evaluation of permanent and primary enamel and dentin mineral density using micro-computed tomography. Oral Radiology, 35(1), 29-34.

Hofmann, P., Marschallinger, R., Daxner-Höck, G. (2009). 3D volume modelling of fossil small mammal teeth using micro CT and object based image analysis. In: Computational Vision and Medical Image Processing. Tavares J. and Jorge N.,(Eds.)(CRC Press/Taylor & Francis) pp. 395–399.

Iwaka, Y. (2006) Three-dimensional observation of the pulp cavity of mandibular first molars by micro-CT. Journal of Oral Biosciences, 48(2), 94–102.

Karaaslan, E.S., Ertas, E., Koprulu, H. (2008). Dental hard tissue abrasions and treatments. Journal of Ondokuz May?s Univ Fac Dent, 9, 28–34.

Keles, A., Alcin, H. (2015). Micro Computed tomography and its aplications in endodontics. Türkiye Klin J Endod, 1(3), 32–39.

Ketterl, W. (1983). Age-induced changes in the teeth and their attachment apparatus. International Dental Journal, 33(3), 262–271.

Kim, I., Paik, K-S., Lee, S-P. (2007). Quantitative evaluation of the accuracy of micro-computed tomography in tooth measurement. Clinical Anatomy, 20(1), 27–34.

Kim, Y.J., Henkin, J. (2015). Micro-computed tomography assessment of human alveolar bone: bone density and three-dimensional microarchitecture. Clinical Implant Dentistry and Related Research, 17(2), 307–313.

Kinney, J.H., Marshall, G.W., Marshall, S.J. (1994). Threedimensional mapping of mineral densities in carious dentin: theory and method. Scanning Microscopy, 8(2), 197–204.

Kurt, M.H., Orhan, K. (2016). Micro-Computed Tomography in Dentistry. Turkiye Klin Oral Maxillofacial Radiology - Spec Top, 2, 14–21.

Ma, J-L., Shi, S-Z., Ide, Y., Saka, H., Matsunaga, S., Agematsu, H. (2013). Volume measurement of crowns in mandibular primary central incisors by micro-computed tomography. Acta Odontologica Scandinavica, 71(5), 1032–1037.

Marciano, M., Duarte, M., Ordinola-Zapata, R., Del Carpio-Perochena, A., Cavenago, B., Villas Bôas, M., et al. (2012). Applications of Micro-Computed Tomography in Endodontic Research. In: A. Mendez, pp. 782–788.

Nakata, K., Nikaido, T., Nakashima, S., Nango, N., Tagami, J. (2012). An approach to normalizing micro-CT depth profiles of mineral density for monitoring enamel remineralization progress. Dental Materials Journal, 31(4), 533–540.

Nanci, A. (2003). Ten Cate’s Oral Histology: Development, Structure, and Function. 6. Ed. St. Louis: Mosby Elsevier, 79-110,145-195.

N.V. (2005). SksScan 1172 X-Ray Microtomography User Manual. V lunchtenburgstraat: Aartselaar.

Oi ,T., Saka, H., Ide, Y. (2004). Three-dimensional observation of pulp cavities in the maxillary first premolar tooth using micro-CT. International Endodontic Journal, 37(1), 46–51.

Olejniczak, A.J., Tafforeau, P., Feeney, R.N.M., Martin, L.B. (2008). Three-dimensional primate molar enamel thickness. Journal of Human Evolution, 54(2), 187–195.

Philippas, G.G. (1961) Influence of Occlusal Wear and Age on Formation of Dentin and Size of Pulp Chamber. Journal of Dental Research, 40(6), 1186–1198.

Sahin, F.U., Topuz, O. (2014). Micro computerized tomography applications in dental research. Acta Odontologica Turcica, 31(2), 114–120.

Schmitz, J.E., Teepe, J.D., Hu, Y., Smith, C.E., Fajardo, R.J., Chun, Y-H.P. (2014). Estimating mineral changes in enamel formation by ashing/BSE and microCT. Journal of Dental Research, 93(3), 256–262.

Someda, H., Saka, H., Matsunaga, S., Ide, Y., Nakahara, K., Hirata, S., et al. (2009). Age estimation based on three-dimensional measurement of mandibular central incisors in Japanese. Forensic Science International, 185(1-3), 110–114.

Uchiyama, T., Tanizawa, T., Muramatsu, H., Endo, N., Takahashi, H.E., Hara, T. A. (1997). Morphometric Comparison of Trabecular Structure of Human Ilium Between Microcomputed Tomography and Conventional Histomorphometry. Calcified Tissue International, 61(6), 493–498.

Weidmann, S.M., Weatherellm J.A., Hamm, S.M. (1967). Variations of enamel density in sections of human teeth. Archives of Oral Biology, 12(1), 85–97.

Wong, F.S.L., Anderson, P., Fan, H., Davis, G.R. (2004). Xray microtomographic study of mineral concentration distribution in deciduous enamel. Archives of Oral Biology, 49(11), 937–944.

Zou, W., Hunter, N., Swain, M.V. (2011). Application of Polychromatic µCT for Mineral Density Determination. Journal of Dental Research, 90(1), 18–30.

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Published

2022-07-30

How to Cite

Yildiz, S., Yavuz, I., Kamalak, H., & Akkus, Z. (2022). Assessing the feasibility of micro-computed tomography in comparing mineral densities and volume values of enamel and dentine in permanent premolars which were extracted teeth for orthodontic and periodontal treatment . IIUM Journal of Orofacial and Health Sciences, 3(2), 172–180. https://doi.org/10.31436/ijohs.v3i2.138