Wound contraction and epithelisation effects of Acrostichum aureum L. in rabbits
Keywords:Excisional wound, mangrove, wound healing, tannin content
Introduction: Rhizomes paste from Acrostichum aureum Linné was used traditionally by Malays ethnic groups in Malaysia for wound healing treatment. To evaluate wound healing properties of aqueous and ethanolic extract of A. aureum on rabbits. Materials and methods: There were four treatments namely aqueous extracts of rhizomes, leaves and stems A. aureum with low and high dose (5 % and 10 %). There were four rabbits in three treatment groups with each individual were inflicted with excisional wounds on their back near the neck with 6 mm in diameter. Topical treatment was applied once daily until complete healing with Solcoseryl jelly served as positive control group and blank aqua cream served as negative control group. The percentage period of epithelisation and wound contraction were measured every 3 days interval. At day 15, all healed wound specimens were biopsied and stained with Masson’s trichrome staining for histopathological study. Results and discussion: From the results, all extracts from A. aureum possessed tannins and total tannin content showed that ethanolic extracts had higher total tannin content compared to aqueous extracts. Based on percentage wound contraction and epithelisation period, the treatment with 5 % aqueous extracts of leaves A. aureum was the most effective wound healing agent in enhancing higher percentage wound contraction, rapid epithelisation period, producing more collagens and fibroblasts proliferation. Conclusion: It was suggested that wound healing properties of rhizomes and leaves A. aureum was contributed by its high total tannin content. This finding would be able to justify its traditional claim as wound healing treatment by Malays communities in Malaysia.
Agyare C, Lechtenberg M, Deters A, Petereit F, Hensel A. (2011). Ellagitannins from Phyllanthus muellerianus (Kuntze) Exell.: geraniin and furosin stimulate cellular activity, differentiation and collagen synthesis of human skin keratinocytes and dermal fibroblasts. Phytomedicine. 18(7), 617-624.
Bandaranayake WM. (1998). Traditional and medicinal uses of mangroves. Mangroves and Salt Marshes, 2, 133–148.
Bandaranayake WM. (1999). Economic, traditional and medicinal uses of mangroves.AIMS, 24-26.
Bandaranayake WM, (2002). Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetlands Ecology and Management, 421–452.
Benjamin A, Manickam VS. (2007). Medicinal pteridophytes from the Western Ghats.Indian J. Traditional Knowledge, 6 (4), 611-618.
Defilipps RA, Maina SL, Pray LA. (1988). The Palauan and Yap medicinal plant studies of
Masayoshi Okabe 1941-1943. Atoll Research Bulletin, 317, 1-25.
Getie M, Gebre M T, Reitz R, Neubert RH. (2002). Evaluation of the release profile of flavonoids from topical formulations of the crude extract of the leaves of Dodonea viscosa (Spindaceae). Pharmazie, 57, 320-22.
Giesen W, Wulffraat S, Zieren M, Scholten. (2006). Mangrove guidebook in Southeast Asia. FAO & Wetlands International, 264-267.
Goss RJ, (1992). Regeneration versus repairs. In: Cohen K, Diegelmann RF, Lindblad WJ.
Wound healing: biochemical & clinical aspects. W. B. Saunders Company, Philadelphia.
Haile, M., & Kang, W. (2019). Antioxidant Activity, Total Polyphenol, Flavonoid and Tannin Contents of Fermented Green Coffee Beans with Selected Yeasts. Fermentation, 5(1), 29. https://doi.org/10.3390/fermentation5010029
Herman. HP, Susanti, D. Saad, S, Taher,M. Ramli, N (2012). In vitro evaluation of wound healing properties of fractions and extracts of Acrostichum aureum Linné and A. speciosum Willd. Proceedings of International Science Postgraduate Conference 2012 (ISPC 2012), Skudai: UTM.
Hemayet H, Ismet AJ, Ishrat N, Musfizur MH, Sariful Islam H, Sk Moniruzzaman. (2012).
Antidiarrhoeal activity and total tannin content from the root of Acrostichum aureum (Linn.). Int J Pharmascholars, 1 (1), 81-85.
Herman, H. P., Susanti, D., Saad, S., Taher, M., & Ramli, N. (2013). Wound Healing Properties of Ethanolic Extract of Acrostichum aureum and Acrostichum speciosum Rhizome in Rats. Journal of Tropical Resources and Sustainable Science (JTRSS), 1(2), 42–48.
Hong PH, San, H. T., 1993. Mangroves of Vietnam. IUCN, 13-16.
Hossan MS, Hanif A, Agarwala B, Sarwar MS, Karim M, Rahman MT, Rahmatullah M. (2010).
Traditional use of medicinal plants in Bangladesh to treat urinary tract infections and sexually transmitted diseases. Ethnobotany Research & Pub, 8, 61-74.
Hout S, Cheab A, Bunb SS, Elias R, Gasquet M, David PT, Azas N. (2006). Screening of selected indigenous plants of Cambodia for antiplasmodial activity. J Ethnopharmacol, 107, 12-18.
Ionita L, Braticevici CF, Tanase A, Ionita C, Campeanu G, Ivana S. (2009). Preliminary experiment in the healing acceleration and tissue recovery in animals (rabbits) with the application in the plague of an extract of Benincasa hyspida. Romanian Biotechnol Lett, 14(4), 4597-4605.
Kathiresan K, Rajendran N. (2005). Mangrove ecosystems of the Indian Ocean region. Indian J Mar Sci, 34 (31), 104-113.
Li K, Diao Y, Zhang H, Wang S, Zhang Z, Yang H. (2011). Tannin extracts from immature fruits of Terminalia chebula Fructus Retz. promote cutaneous wound healing in rats. BMC Complement Altern Med, 11(86), 1-9.
Ling T, Kirsner RS, Jie L. (2011). Extracellular matrix molecules in skin wound repair. In:Middleton JE. Wound Healing: Process, Phases, and Promoting. New York: Nova Science Publishers Inc, 73-93.
Mahmood AA, Phipps ME. (2006). Wound healing activities of Plantago major leaf extracts in rats. Intl J Tropical Med, 1 (1): 33-35.
Mahmood AA, Khaled AA, Hapipah MA. Suzita MN, Salmah I. (2009). Wound healing activities of Rafflesia Hasseltii extract in rats. J Clin Biochem Nutr, 45, 304- 308.
Mannan MM, Maridass M, Victor B. (2008). A review on the potential uses of ferns. Ethnobotanical leaflets, 12, 281-285.
Ministry of Science, Environment and Technology. 1998. Malaysia’s National Policy on Biological Diversity. Available at http://www.arbec.com.my. Accessed on 1 April 2013.
Moghbel A, Hemmati AAH, Agheli H, Rashidi I, Pharm KA. (2005). The effect of tragacanth mucilage on the healing of full thickness wound in rabbits. Arch Iranian Med, 8(4), 257-262.
Moon EJ, Lee YM, Lee OH, Lee MJ, Lee SK, Chung MH, Park YI, Sung CK, Choi JS, Kim
KW. (1999). A novel angiogenic factor derived from Aloe vera gel: beta-sitosterol, a plant sterol. Angiogenesis, 3, 117–123.
Ong HC. (2004). Tumbuhan liar: khasiat ubatan & kegunaan lain. Utusan Publications & Distributors Sdn. Bhd., Kuala Lumpur:
Rahmatullah M, Hossan MS, Abu Hanif, Roy P, Jahan R, Mujib Khan, Chowdury MH, Rahman T. (2009). Ethnomedicinal applications of plants by the traditional healers of
the Marma tribe of Naikhongchhari, Bandarban district, Bangladesh. Advances in Natural and Applied Sciences, 392-401.
Smith AR, Pryer KM, Schuettpelz E, Korall P, Schneider H, Wolf PG. (2006). A classification of extant ferns. Taxonomy, 55(3), 705-731.
Suvik A, Effendy AWM. (2012). The use of modified Masson’s trichrome staining in collagen evaluation in wound healing study. Malays J Vet R, 3(1), 39-47.
Thi Minh, T., Thu, N. H., Toan, H. K., Huong, T. T., Quang, T. T., Thanh, N. T. V., & Sung, T. V. (2021). Three New Phenolic Sulfates from Acrostichum aureum Collected from Coastal Area of Thai Binh Province, Vietnam and Their Cytotoxic Activity. Records of Natural Products, 1,66–73. https://doi.org/10.25135/rnp.252.21.03.2014
Wu, X., Huang, Q., Xu, N., Cai, J., Luo, D., Zhang, Q., Su, Z., Gao, C., & Liu, Y. (2018). Antioxidative and Anti-Inflammatory Effects of Water Extract of Acrostichum aureum Linn. Against Ethanol-Induced Gastric Ulcer in Rats. Evidence-Based Complementary and Alternative Medicine, 2018, 1–10. https://doi.org/10.1155/2018/3585394
Xie EF, Xu RX, Wang CC, Pu ZB, Liusup HB, Zhou LG., Ge JL. (2002). The effect of moist exposed burn ointment on maintaining a physiological moist environment in treating burn wound. Ann Burns Fire Disasters, 15(3), 102-124.
Young B, Lowe JS, Stevens A, Heath JW, (2006). Wheater’s functional histology; a text and colour atlas. Philadephia: Churchill Livingstone.
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