Tualang Honey Supplementation Alleviates Obesity and Dyslipidaemia in High Cholesterol Diet Induced Non-Alcoholic Steatohepatitis Animal Model

Authors

  • Mohd Afzal Alias Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
  • Roslina Abdul Rahim Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
  • Siti Aesah @ Naznin Muhamad Department of Pathology and Laboratory Medicine, Kulliyyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
  • Nor Zamzila Abdullah Department of Pathology and Laboratory Medicine Kulliyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
  • Norlelawati A Talib Department of Pathology and Laboratory Medicine Kulliyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia
  • Khairunisa Ahmad Affandi Department of Pathology and Laboratory Medicine Kulliyah of Medicine, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.31436/imjm.v21i4.2107

Keywords:

Non-alcoholic steatohepatitis, Tualang honey, Obesity, Dyslipidaemia, Animal model

Abstract

INTRODUCTION: Dyslipidaemia and obesity are two main features of non-alcoholic steatohepatitis (NASH). This study aimed to investigate the effects of Tualang honey (TH) supplementation on bodyweight, liver weight, and lipid profiles in high cholesterol diet (HCD) induced NASH animal model. MATERIALS AND METHODS: Sixteen Sprague-Dawley rats were given 12% HCD for 16 weeks to induce NASH. These animals were divided into 4 groups; Group 1 (continued HCD), Group 2 (changed to normal diet), Group 3 (normal diet and TH 1.2g/kg) and Group 4 (normal diet and TH 3.0g/kg) for the following 4 weeks. Bodyweight was measured daily. At the end of the study, blood was collected via retro-orbital bleeding and the rats were sacrificed to harvest their liver. RESULTS: The group 4 rats had significantly lower mean final bodyweight than rats in group 1, 2 and 3 (478.0±24.4 vs. 641.5±25.1, 593.8 ±29.3, 552.0±72.9 g, p<0.05). Animals in group 4 were also found to have a significantly lower mean liver weight compared to groups 1 and 2 (12.9±0.9 vs 20.1±2.2, 15.7±1.2 g, p<0.05). In comparison to controls, the mean concentration of total cholesterol was significantly lower in all the other groups and the lowest mean concentration of triglycerides was recorded in group 4 with significant difference when compared to the controls (0.9±0.4 vs 3.6±0.4 mmol/L, p<0.05). CONCLUSION: The change from HCD to a normal diet coupled with TH supplementation has been shown to reduce bodyweight, liver weight, total cholesterol and triglyceride levels in the 12% HCD NASH induced animal models.

 

 

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Published

01.10.2022

How to Cite

Alias, M. A., Abdul Rahim, R., Muhamad, S. A. @ N., Abdullah, N. Z., A Talib, N., & Ahmad Affandi, K. (2022). Tualang Honey Supplementation Alleviates Obesity and Dyslipidaemia in High Cholesterol Diet Induced Non-Alcoholic Steatohepatitis Animal Model. IIUM Medical Journal Malaysia, 21(4). https://doi.org/10.31436/imjm.v21i4.2107

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