PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OPTIMIZATION OF TRIGONA HONEY AND PROPOLIS USING RESPONSE SURFACE METHODOLOGY

Authors

  • Abdullah Hagar Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
  • Muhammad Ibrahim Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia
  • Nurlisa Fatihah Abd Rani Department of Nutrition Sciences, Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia

Abstract

The optimum combination of honey and propolis for total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity were analysed for Trigona honey and Trigona propolis aqueous extract using response surface methodology and central composite design. The influence of honey (X1:15–16.5 g) and propolis (X2:13.5–15g) on TPC (Y1), TFC (Y2), DPPH (Y3), ABTS (Y4) and FRAP (Y5) were tested. The experimental results were adequately fitted into a second-order polynomial model regarding TPC (R2 = 0.9539, p =0.0002), TFC (R2 = 0.9209, p = 0.0010), DPPH (R2 = 0.9529, p =0.0002), ABTS (R2 = 0.9817, p < 0.0001), and FRAP (R2 = 0.9363, p = 0.0005). The optimum percentage compositions of honey and propolis were 15.26g (50.43%) and 15g (49.57%), respectively. The predicted outcomes for  TPC, TFC, DPPH(IC50), ABTS, and FRAP were 162.46 mg GAE /100g, 2.29 mg QE/g , 14.52 mg/ml,  564.27 µMTE/g, and 3.56 mMTE/g, respectively. The experimental results were close to predicted outcomes, 152.06 ±0.55 mg GAE/100g, 2.21± 0.05 mg QE/g , 13.85 ± 0.34 mg/ml, 555.22±36.84 µMTE/g, and 3.71± 0.02mMTE/g, respectively. Therefore, the optimum combination of honey and propolis with remarkable antioxidant properties could be applied as food additives, cosmetics and pharmacological applications.

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Published

2021-04-08

How to Cite

Hagar, A. ., Ibrahim, M. ., & Abd Rani, N. F. . (2021). PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY OPTIMIZATION OF TRIGONA HONEY AND PROPOLIS USING RESPONSE SURFACE METHODOLOGY. INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES, 5(1), 2142. Retrieved from https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/579

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