Lupeol Isolation and Hepatoprotective Activity of the Methanol Aerial Parts Extract of Indigofera Stenophylla (Fabaceae)
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Abstract
Introduction: Indigofera stenophylla Guil. and Perr. (Fabaceae) is a medicinal plant used in traditional medicine for the treatment of diseases. This study aimed to isolate phytoconstituent(s) and establish the hepatoprotective activity of the methanol aerial parts extract of Indigofera stenophylla (MAEIS). Methods: The plant material of 1500 g was extracted with 70% methanol using a maceration method, and the extract was partitioned respectively into hexane and chloroform fractions (HF and CF). The HF was subjected to column chromatographic techniques to afford 5 mg of compound AR1 characterized using 1H and 13C-NMR. A single dose of 7,12 Dimethylbenz(a)anthracene was administered orally at 30 mg/kg body weight, followed by 28 days treatment with graded doses of MAEIS and Silymarin. Blood samples were collected and analyzed for Aspartate Amino Transaminase (ASP), Alanine Amino Transaminase (ALT) and Total Protein (TP) assays. The livers were harvested for histopathological examination. Results: The isolated compound AR1 was elucidated as lup-20(29)-en-3-ol (lupeol). The MAEIS group at dose of 600 mg/kg and silymarin 200 mg/kg body weight respectively lowered the levels of AST and ALT significantly (p<0.05) indicating a reduction in liver damage. The MAEIS increased the total protein levels and normal histopathological profile of the liver tissue. Conclusion: This is the first isolation of lupeol from MAEIS. MAEIS provides a scientific basis for its ethnomedicinal uses and demonstrated significant hepatoprotective activity.
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References
Adeshina, A., Hassan, H. S., Sani, Y. M., Jimoh, A. A., Bakare, L. O., & Sadam, A. A. (2021). Isolation and characterization of lupeol and stigmasterol from methanol root extract of Combretum hypopolinum (Diels.) Okafor (Combretaceae). Journal of Pharmaceutical and Allied Sciences, 18(4), 3543–3549.
Agbaje, E. O., Adeneye, A. A., & Daramola, A. O. (2019). Biochemical and toxicological studies of aqueous extract of Syzygium aromaticum (L.) Merr. and Perry (Myrtaceae) in rodents. African Journal of Traditional Complementary and Alternative Medicines, 6, 214–254. https://doi.org/10.4314/ajtcam.v6i3.57162
Agbaje, E. O., Adeneye, A. A., & Daramola, A. O. (2019). Biochemical and toxicological studies of aqueous extract of Syzygium aromaticum (L.) Merr. and Perry (Myrtaceae) in rodents. African Journal of Traditional Complementary and Alternative Medicines, 6, 214–254. https://doi.org/10.4314/ajtcam.v6i3.57162
Ahmed, T., & Sadaf, F. (2025). Phytopharmacological properties of Indigofera heterantha var. gerardiana (Wall. ex Brandis) Baker: A comprehensive review. International Journal of Advanced Research, 13(5), 50-66. https://doi.org/10.21474/IJAR01/20876
Allameh, A., Niayesh-Mehr, R., Aliarab, A., Sebastiani, G., & Pantopoulos, K. (2023). Oxidative stress in liver pathophysiology and disease. Antioxidants, 12(9), 1653. https://doi.org/10.3390/antiox12091653
Anas, H. (2018). Isolation of lupeol from the methanol leaf extract of Hymenocardia acida Tul. (Euphorbiaceae). Journal of Pharmaceutical and Allied Sciences.
Arora, R., Bhushan, S., Kumar, R., Manna, R., Kaur, P., Singh, A. P., Sigh B., Vig, A. P., Sharma, D., and Arora S. (2014). Hepatic Dysfunction Induced by 7, 12-dimethylbenz(a)anthracene and its obviation with erucin using enzymatic and histological changes as indicators, PLOS ONE, 9(11): e112614
Bhagavan, N. B., Arunachalam, S., Dhasarathan, P., & Kannan, N. D. (2013). Evaluation of anti-inflammatory activity of Indigofera aspalathoides Vahl in Swiss albino mice. Journal of Pharmacy Research, 6, 350–354.
Campos, J. K. L., Araújo, T. F. da S., Brito, T. G. da S., da Silva, A. P. S., da Cunha, R. X., Martins, M. B., da Silva, N. H., dos Santos, B. S., da Silva, C. A., & de M. Lima, V. L. (2018). Indigofera suffruticosa Mill. (Anil): Plant profile, phytochemistry, and pharmacology review. Advances in Pharmacological Sciences, Article ID 8168526, 1-13. https://doi.org/10.1155/2018/8168526
Fernando, I., & Soysa, P. (2014). Total phenolic, flavonoid contents, in-vitro antioxidant activities and hepatoprotective effect of aqueous leaf extract of Atalantia ceylanica. BMC Complementary and Alternative Medicine, 14, 395. https://doi.org/10.1186/s12906-014-0395-0
Gerometta, E., Grondin, I., Smadja, J., Frederich, M., & Gauvin-Bialecki, A. (2019). A review of traditional uses, phytochemistry and pharmacology of the genus Indigofera. Journal of Ethnopharmacology, 255, Article 112, 1-25. https://doi.org/10.1016/j.jep.2019.112667
Ipav, S. S., Igoli, J. O., Tor-Anyiin, T. A., & Anyam, J. V. (2022). Isolation and characterization of lupeol and lupeol acetate from propolis obtained from Benue State. Journal of Chemical Society Nigeria, 47(1), 152–159.
Lakshmana Rao, S. K., Aminabee, S., & Chinna Eswaraiah, M. (2018). Evaluation of hepatoprotective activity of Indigofera barberi in rats against paracetamol induced hepatic injury. Advances in Investigational Pharmacology and Therapeutic Medicine, 1, 1-9.
Longe, S. A., Mutiat Adetayo, Omotayo Oyinlade, Cecilia Ogundare, & Adeteju Oluwafunmilayo. (2015). Hepatoprotective effect of Mangifera indica stem bark extracts on paracetamol-induced oxidative stress in albino rats. European Scientific Journal, 11(24), 1-9.
Ma, X., Huang, T., Chen, X., Li, Q., Liao, M., Fu, L., Huang, J., Yuan, K., Wang, Z., & Zeng, Y. (2025). Molecular mechanisms in liver repair and regeneration: From physiology to therapeutics. Signal Transduction and Targeted Therapy, 10, 63. https://doi.org/10.1038/s41392-024-02104-8
Mailafiya, M. M., Pateh, U. U., Hassan, H. S., Sule, M. I., Bila, A. H., Musa, T. L., Atinga, V., & Achika, J. I. (2020). Isolation of lupeol from the stem bark of Leptadenia hastata (Pers.) Decne. Journal of Applied Sciences and Environmental Management, 24(10), 1835–1838. https://doi.org/10.4314/jasem.v24i10.19
Mary, A., & Chatterjee, P. C. (2006). Hepatoprotective effect of the aqueous extract of Phyllanthus niruri on nimesulide-induced oxidative stress in vivo. International Journal of Biochemistry and Biophysics, 43, 299-305.
Muktar, B., Bello, I. A., & Sallau, M. S. (2018). Isolation, characterization and antimicrobial study of lupeol acetate from the root bark of Fig-Mulberry Sycamore (Ficus sycomorus LINN). Journal of Applied Science and Environmental Management, 22(7), 1129–1133. https://doi.org/10.4314/jasem.v22i7.21
Murshed, M., Al-Tamimi, J., Aljawdah, H. M. A., Qaid, M. M., & Al-Quraishy, S. (2025a). Phytochemical analysis and antioxidant activities of Indigofera oblongifolia, local plant extract used in traditional medicine: Antimalarial activities. Bol Latinoam Caribe Plant Med Aromat, 24(1), 62-75. https://doi.org/10.37360/blacpma.25.24.1.5
Murshed, M., Al-Tamimi, J., Aljawdah, H. M. A., Qaid, M. M., & Al-Quraishy, S. (2025b). Antimicrobial activity and phytochemical analysis of Indigofera tinctoria. International Journal of Advanced Research, 13(5), 50-66. https://doi.org/10.21474/IJAR01/20876
Omprakash, K. K., Chandran, G. S., & Velpandian, V. (2013). Indigofera aspalathoides Vahl Ex DC (Sivanar vembu): A phyto pharmacological review. International Journal of Pharmaceutical Sciences and Research, 4(10), 3775-3781. https://doi.org/10.13040/IJPSR.0975-8232.4(10).3775-81
Premalatha, D. S., Tangavelou, A. C., & Kamaraj, R. (2015). Hepatoprotective activity of Indigofera longiracemosa (Leguminosae). International Journal of Modern Research and Reviews, 3(1), 608-611.
Rahim et al. (2014). African Journal of Traditional Complementary and Alternative Medicines, 11(2), 447–451. https://doi.org/10.4314/ajtcam.v11i2.12.
Rahman, T. U., Zeb, M. A., Liaqat, W., Sajid, M., Hussain, S., & Choudhary, M. I. (2018a). Phytochemistry and pharmacology of genus Indigofera: A review. Rec. Nat. Prod., 12(1), 1-13. https://doi.org/10.25135/rnp.13.16.12.585
Rahman, T. U., Zeb, M. A., Liaqat, W., Sajid, M., Hussain, S., & Choudhary, M. I. (2018b). Phytochemistry and pharmacology of genus Indigofera: A review. Rec. Nat. Prod., 12(1), 1-13. https://doi.org/10.25135/rnp.13.16.12.585
Sanusi, A., Hassan, H. S., Sani, Y. M., Akinpelu, L. A., Muhammad, H., Ado, Y. M., Tomilayo, R. B., Yusuf, A. J., Muntaka, A., Echu, F. A., Muhammad, S., Njinga, N. S., Abdullahi, S. T., Ayanniyi, R. O., & Bakare-Odunola, M. T. (2025b). Stigmasterol isolation and anticonvulsant activity of methanol extract from the aerial parts of Indigofera stenophylla (Fabaceae). Bulletin of Pharmaceutical Sciences, 48(2).
Sanusi, A., Hassan, H. S., Sani, Y. M., Musa, A. M., Pateh, U. U., Magaji, M. G., Mahmoud, M. A., Yusuf, A. J., & Mailafiya, M. M. (2018). Preliminary phytochemical and anticonvulsant studies on the root extracts of Ficus capensis Thunb. (Moraceae). Journal of Pharmacy and Bioresources, 15(1), 1-9. https://doi.org/10.4314/jpb.v15i1.1
Sanusi, A., Yahaya, M. K., Hassan, H. S., Magaji, M. G., Musa, A. M., Yusuf, A., Evuty, A., Muhammad, Z., Njinga, N. S., Bakare-Odunola, M. T., Abdullahi, S. T., Dauda, G. (2025a). Phytochemical and antiproliferative effect of Indigofera stenophylla Guil. and Perr. (Fabaceae) against 7,12-dimethylbenzanthracene induced breast tumor in rats. Tropical Journal of Natural Product Research, 9(3), 1248–1255. http://www.tjnpr.org
Sanusi, A., Yahaya, M. K., Hassan, H. S., Musa, A. M., Magaji, M. G., Muntaka, A., Njinga, N. S., Ismail, S., Garba, D., Yusuf, A. J., & Echu, F. (2024). Isolation and characterization of 5-hydroxy-rutin-6,7-dimethyl ether and cilicicone ? flavonoids from Indigofera stenophylla Guil. and Perr. Nigerian Journal of Scientific Research, 23(2), 299-310. https://njsrabuzaria.com.ng
Sawale, J. A., Patel, J. R., & Kori, M. L. (2017). Isolation and characterization of oleonolic acid and lupeol from Vitex negundo leaves. Journal of Pharmacognosy and Phytochemistry, 6(4), 936–938.
Srivastava, A., & Shivanandappa, T. (2010). Hepatoprotective effect of the root extract of Decalepis hamiltonii against carbon tetrachloride-induced oxidative stress in rats. Food Chemistry, 118, 411-417