Evaluating the effect of Tualang honey on the viability and migration of human gingival fibroblasts
DOI:
https://doi.org/10.31436/ijohs.v7i1.425Keywords:
human gingival fibroblast, Tualang honey, viability and migration, wound healingAbstract
Tualang honey, produced by the wild bee species Apis dorsata, is predominantly found in the South Asian region, particularly in Malaysia. Tualang honey has attracted scientific interest for its antimicrobial, anti-inflammatory, and wound-healing properties, highlighting its potential for therapeutic use. The study aimed to assess the effects of Tualang honey on the viability and migration of human gingival fibroblasts (HGFs) using MTT and scratch assays. HGFs were cultured in standard fibroblast medium at 37°C in a 5% CO2 humidified incubator. Cells were seeded at 5×10³ cells/well in a 96-well plate and incubated for 24 hours before being treated with varying concentrations of Tualang honey, with the negative control receiving only growth medium. After 24 hours, cell viability was assessed using the MTT assay and absorbance was measured at 570 nm. For the scratch migration assay, cells were seeded at 10×10³ cells/well in a 24-well plate and incubated for 24 hours. A scratch was made on the cell layer, followed by treatment with Tualang honey at various concentrations, a negative control (untreated cells), and a positive control (0.1% Gengigel mouthwash). Images were captured at 0-, 24-, and 48-hours using Image-Pro Express software. This study demonstrated that Tualang honey at concentrations of 2.5% and below promoted high cell viability (above 88%), while concentrations ?0.3% significantly enhanced cell migration. These findings indicate that Tualang honey, when applied at appropriate concentrations, is non-cytotoxic and promotes cell proliferation and possesses strong potential for periodontal tissue healing applications. Therefore, it shows potential as a natural agent for enhancing periodontal wound healing.
References
Abu Bakar, M. F., Sanusi, S. B., Abu Bakar, F. I., Cong, O. J., & Mian, Z. (2017). Physicochemical and antioxidant potential of raw unprocessed honey from malaysian stingless bees. Pakistan Journal of Nutrition, 16(11), 888–894. https://doi.org/10.3923/pjn.2017.888.894
Aspar, M. M., Edros, R., & Hamzah, N. A. (2020). Antibacterial evaluation of malaysian kelulut, tualang and acacia honey against wound infecting bacteria. IOP Conference Series: Materials Science and Engineering, 991(1). https://doi.org/10.1088/1757899X/991/1/012065
Azman, A.S.S., Tan, J.J., Abdullah, M.N.H., et al. (2024). Medicinal activities of Tualang honey: a systematic review. BMC Complementary Medicine and Therapies, 24 (266).
Bansal, V., Medhi, B., & Pandhi, P. (2005). Honey--a remedy rediscovered and its therapeutic utility. Kathmandu University Medical Journal (KUMJ), 3(3), 305–309.
de Luna, F. C., Ferreira, W. A., Casseb, S. M., & de Oliveira, E. H. (2023). Anticancer potential of flavonoids: an overview with an emphasis on tangeretin. Pharmaceuticals, 16 (9). https://doi.org/10.3390/ph16091229
Ezz El-Arab, A. M., Girgis, S. M., Hegazy, E. M., & Abd El-Khalek, A. B. (2006). Effect of dietary honey on intestinal microflora and toxicity of mycotoxins in mice. BMC Complementary and Alternative Medicine, 6(1), 6. https://doi.org/10.1186/1472-6882-6-6
Fauzi, A.N., Norazmi, M.N., Yaacob, N.S. (2016) Tualang honey promotes apoptotic cell death induced by tamoxifen in breast cancer cell lines. Evidence-Based Complementary and Alternative Medicine, 2016:3729051. https://doi.org/10.1155/2016/3729051
Gharzouli, K., Amira, S., Gharzouli, A., & Khennouf, S. (2002). Gastroprotective effects of honey and glucose-fructose-sucrose-maltose mixture against ethanol-, indomethacin-, and acidified aspirin-induced lesions in the rat. Experimental and Toxicologic Pathology, 54(3), 217–221. https://doi.org/10.1078/0940-2993-00255
Hofmann, E., Fink, J., Pignet, A.-L., Schwarz, A., Schellnegger, M., Nischwitz, S. P., Holzer-Geissler, J. C. J., Kamolz, L.-P., & Kotzbeck, P. (2023). Human in vitro skin models for wound healing and wound healing disorders. Biomedicines, 11 (4). https://doi.org/10.3390/biomedicines11041056
Liang, C., Park, A. Y., & Guan, J. (2007). In vitro scratch assay?: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329–333. https://doi.org/10.1038/nprot.2007.30
Mandal, M. D., & Mandal, S. (2011). Honey: its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine, 1(2), 154–160. https://doi.org/10.1016/S2221-1691(11)60016-6
Marques, A. F. S., Silva, N. M., da Cruz, M., Marques, J., & da Mata, A. D. (2024). Hyaluronic acid-based gels for oral application: comparison of in vitro effects on gingival cells and bacteria. Journal of Oral Biology and Craniofacial Research, 14(3), 238–244. https://doi.org/10.1016/j.jobcr.2024.03.001
Medhi, B., Puri, A., Upadhyay, S., & Kaman, L. (2008). Topical application of honey in the treatment of wound healing: a metaanalysis. JK Science, 10(4), 166–169.
Mercan, U., & Salkin, H. (2019). Comparison of the effect of postoperative care agents on human gingival fibroblasts: a preliminary study. European Oral Research, 53(2), 67–73. https://doi.org/10.26650/eor.20197456
Molan, P.C. (2001). The potential of honey to promote oral wellness. General Dentistry, 49(6), 584–589.
Molan, P.C. (1992). The antibacterial activity of honey. Bee World, 73(1), 5–28. https://doi.org/10.1080/0005772X.1992.11099109
Naruishi, K. (2022). Biological roles of fibroblasts in periodontal diseases. Cells, 11(21). https://doi.org/10.3390/cells11213345
Ranzato, E., Martinotti, S., & Burlando, B. (2012). Epithelial mesenchymal transition traits in honey-driven keratinocyte wound healing: comparison among different honeys. Wound Repair and Regeneration, 20(5), 778–785. https://doi.org/10.1111/j.1524475X.2012.00825.x
Ranzato, E., Martinotti, S., & Burlando, B. (2013). Honey exposure stimulates wound repair of human dermal fibroblasts. Burns & Trauma, 1(1), 32–38. https://doi.org/10.4103/2321-3868.113333
Tan, H. T., Rahman, R. A., Gan, S. H., Halim, A. S., Hassan, S. A., Sulaiman, S. A., et al. (2009). The antibacterial properties of Malaysian tualang honey against wound and enteric microorganisms in comparison to manuka honey. BMC Complementary and Alternative Medicine, 9, 34. https://doi.org/10.1186/1472-6882-9-34
te Boekhorst, V., Preziosi, L., & Friedl, P. (2016). Plasticity of cell migration in vivo and in silico. Annual Review of Cell and Developmental Biology, 32(1), 491–526. https://doi.org/10.1146/annurev-cellbio-111315-125201
Tonks, A. J., Cooper, R. A., Jones, K. P., Blair, S., Parton, J., & Tonks, A. (2003). Honey stimulates inflammatory cytokine production from monocytes. Cytokine, 21(5), 242–247. https://doi.org/10.1016/s1043-4666(03)00092-9
Yaghoobi, R., Kazerouni, A., & Kazerouni, O. (2013). Evidence for clinical use of honey in wound healing as an anti-bacterial, anti-inflammatory anti-oxidant and anti-viral agent: a review. Jundishapur Journal of Natural Pharmaceutical Products, 8(3), 100–104. https://doi.org/10.17795/jjnpp-9487
Yu, L. X., Taib, H., Berahim, Z., Ahmad, A., & Zainuddin, S. L. A. (2015). The effect of Tualang honey on human periodontal ligament fibroblast proliferation and alkaline phosphatase level. Sains Malaysiana, 44(7), 1021–1025. https://doi.org/10.17576/jsm-2015-4407-14
Yun, C. Z. (2021). Cytotoxic evaluation of malaysian kelulut honey on human gingival fibroblast cell line using mtt assay cytotoxic evaluation of Malaysian kelulut honey on human gingival fibroblast. Journal of Dentistry Indonesia, 28(2), 88–93.
Downloads
Published
How to Cite
Issue
Section
