STEP BY STEP PREPARATION OF URINE SAMPLES FOR IN-SOLUTION, GEL-FREE PROTEOMIC STUDIES, SUITABLE FOR MALDI TOF MS AND LCMS QTOF MS

Authors

  • Aniza Pakeer Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia.
  • M. Yusri Idorus Institute for Medical Molecular Biotechnology, University Teknologi Mara Sg. Buloh
  • Nosrihah Ismail Medical Biotechnology Laboratory, Faculty of Medicine, University Malaya Kuala Lumpur
  • Vanitha Mariappa Medical Biotechnology Laboratory, Faculty of Medicine, University Malaya Kuala Lumpur
  • Imad Mustafa al Deen Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia.

Abstract

Two-dimensional electrophoresis is an established method for isolating and separating proteins for proteomic analysis and identification of proteins. However, it requires a lot of optimization and it suffers from some ongoing concerns regarding quantitative reproducibility and limitations on its ability to study certain class of proteins. We propose a simpler method in preparing urine samples from acute melioidosis patients for in-solution proteomics using liquid chromatography coupled with mass spectrometry quadrupole time of flight (LCMS-QTOF MS) or matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). This method is relatively easier than 2-dimensional electrophoresis, affordable, reproducible, can be performed in less equipped laboratories for transport to specialized centers for proteomic studies and bioinformatic analysis.

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Published

2021-04-08

How to Cite

Pakeer, A. ., Idorus, M. Y. ., Ismail, N. ., Mariappa, V. ., & al Deen, I. M. . (2021). STEP BY STEP PREPARATION OF URINE SAMPLES FOR IN-SOLUTION, GEL-FREE PROTEOMIC STUDIES, SUITABLE FOR MALDI TOF MS AND LCMS QTOF MS . INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES, 5(1), 2185. Retrieved from https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/622