VARDENAFIL MARKEDLY SUPPRESSES TUMOR GROWTH AND ENHANCES IDARUBICIN-INDUCED ANTITUMOR EFFECTS AGAINST GLIOMA IN VIVO

Authors

  • ASHOUR A.E. Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia.
  • A. ALZAHRANI Pharmacology Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • H. ABDEL-HAMIED Pathology Department, College of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
  • K. ZOHEIR Cell Biology Department, National Research Center, Dokki, Cairo, Egypt.
  • S. AHMAD Pharmacology Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • S. ALREJAIE Pharmacology Department, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
  • A. ABD-ALLAH Pharmacology Department, College of Pharmacy, Al-Azhar University, Cairo, Egypt.

Abstract

Idarubicin (IDA) is highly potent anticancer against glioma in vitro but not in vivo. Phosphodiesterase (PDE-5) hydrolyses cyclic guanosine monophosphate (cGMP), and is highly expressed in glioma. We proposed that increasing cGMP in the brain by the PDE-5 inhibitor vardenafil (VAR) augments IDA anti-glioma activity. We show that VAR (10 mg/kg) enhanced IDA (0.1mg/kg)-induced tumor growth-inhibitory activity and augmented IDAinduced improvement of survival against rat C6 glioma. Real time PCR results demonstrated that VAR inhibited mRNA expression of PDE-5 in the brains of animals treated with VAR, compared to control-treated group, implicating an important role for the inhibition of PDE-5 in VAR antitumor activity. Animals receiving either VAR alone or VAR+IDA improved survival when compared with control (PBS) or IDA groups.  We examined the antiproliferative, pro-apoptotic and anti-invasive activities VAR in vitro and showed that VAR inhibited the growth and promoted apoptosis of rat C6 glioma cells. VAR also inhibited the migration and anchorage-independent growth of C6 cell. This suggests that the enhanced antitumor effect of VAR could be due to combined antiproliferative, pro-apoptotic and antiinvasive activities.

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Published

2021-04-08

How to Cite

A.E., A. ., ALZAHRANI, A. ., ABDEL-HAMIED, H. ., ZOHEIR, K. ., AHMAD, S. ., ALREJAIE, S. ., & ABD-ALLAH, A. . (2021). VARDENAFIL MARKEDLY SUPPRESSES TUMOR GROWTH AND ENHANCES IDARUBICIN-INDUCED ANTITUMOR EFFECTS AGAINST GLIOMA IN VIVO . INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES, 5(1), 2182. Retrieved from https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/619