PRELIMINARY STUDY ON THE EFFECT OF TUALANG HONEY AND ITS SILVER NANOPARTICLES ON KAINIC ACID-INDUCED MEMORY DEFICITS AND OXIDATIVE DAMAGE IN RAT HIPPOCAMPUS.

Authors

  • Hidani Hasim Department of Chemical Pathology, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia.
  • Sirajudeen Kuttulebbai Naina Mohamed Salam Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia.
  • Pasupuleti Visweswara Rao Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, 88400, Kota Kinabalu, Sabah, Malaysia.
  • Sangu Muthuraju Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston, Houston 77240, TX, United States of America.
  • Muzaimi Mustapha Department of NeuroSciences, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia.
  • Mohd Asnizam Asari Department of Anatomy, School of Medical Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kota Bharu, Kelantan, Malaysia.

Abstract

Kainic acid (KA) mediated excitotoxicity has been shown to cause memory impairment and oxidative stress in rats brain. The aim of this study was to investigate the effect of Tualang honey (TH) and its silver nanoparticles (THSN) on the KA-induced memory deficits and oxidative damage in rats’ hippocampus. Twenty-four adult male Sprague-Dawley rats were randomized into eight groups: (i) control, (ii) THSN (10mg/kg), (iii) THSN (50mg/kg), (iv) KA only, (v) KA+TH, (vi) KA+THSN (10mg/kg), (vii) KA+THSN (50mg/kg), and (viii) KA+Topiramate. Based on their respective groups, rats were pretreated orally with either distilled water, THSN (10 or 50 mg/kg body weight), Tualang honey (1.0 g/kg body weight), or Topiramate (40 mg/kg body weight), five times at 12 hours intervals. Saline or KA (15 mg/kg body weight) were injected subcutaneously 30 min after last oral treatment. Novel object recognition test (NORT) was performed for memory assessment. The rats were sacrificed 24 hours post KA induction and hippocampus was harvested. Malondialdehyde (MDA) and superoxide dismutase (SOD) were measured using commercially available ELISA kits. The results showed that there was a trend of decreased in MDA level in both TH and THSN groups, however there were no significance difference. There was significantly increased level in SOD following pretreatment with TH and THSN groups compared to KA only group. Interestingly, these pretreatment groups also demonstrated enhanced memory as evidenced by significant increase in recognition index in NORT when compared to KA only group. In conclusion, this preliminary finding suggests that the pretreatment with TH and THSN might have potential role to improve the memory and ameliorate oxidative stress in the KA-induced exitotoxicity rats. However, further study needs to be carried out to understand the precise mechanism.

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Published

2021-04-08

How to Cite

Hasim, H. ., Mohamed Salam, S. K. N. ., Rao, P. V. ., Muthuraju, S. ., Mustapha, M. ., & Asari, M. A. . (2021). PRELIMINARY STUDY ON THE EFFECT OF TUALANG HONEY AND ITS SILVER NANOPARTICLES ON KAINIC ACID-INDUCED MEMORY DEFICITS AND OXIDATIVE DAMAGE IN RAT HIPPOCAMPUS. INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES, 5(1), 2158. Retrieved from https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/595