EVALUATION OF WOUND HEALING PROPERTIES OF PLECTRANTHUS AMBOINICUS USING HPdLF FIBROBLAST CELL LINES AS STUDY MODEL

Abstract

Plectranthus amboinicus is a semi-succulent with pungent oregano odour that belongs to family Lamiaceae. It has been extensively used in traditional medicine for its wound-healing effect. However, there is a lack of scientific evidence to support the claimed. Thus, this study aimed to evaluate wound healing properties of P. amboinicus extracts through in vitro study. Six extracts were used in this study, including three parts of the P. amboinicus, namely, leaves, stem and roots with two types of solvent, aqueous and methanol respectively. Cell proliferation assay was conducted to identify a significant dose that has the potential of wound healing properties. Determined concentration then proceeded with a scratch wound assay for evaluation. Based on the result, it shows that methanol root extract of P. amboinicus with concentration 0.3906 µg/ml has a high potential of wound healing properties in comparison with control. These findings would support the potential use of P. amboinicus as a wound-healing plant.