THE EXPRESSION OF MICRORNA-744 (MIR-744) IN NASOPHARYNGEAL CARCINOMA: A PRELIMINARY STUDY

Authors

  • MOHD ARIFIN BIN KADERI Kulliyyah of Allied Health Sciences, International Islamic University Malaysia, Jalan Sultan Ahmed Shah Bandar Indera Mahkota, 25200 Kuantan, Pahang DarulMakmur-Malaysia, Malaysia
  • NOOR SYAMILA BINTI OTHMAN DEPARTMENT OF BIOMEDICAL SCIENCE, KULLIYYAH OF ALLIED HEALTH SCIENCE, INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA (IIUM), PAHANG 25200, MALAYSIA
  • WAN ISHLAH BIN LEMAN DEPARTMENT OF OTORHINOLARYNGOLOGY-HEAD & NECK SURGERY, KULLIYYAH OF MEDICINE, INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA (IIUM) PAHANG 25200, MALAYSIA and OTORHINOLARYNGOLOGY DEPARTMENT, HOSPITAL TENGKU AMPUAN AFZAN, KUANTAN, JALAN TANAH PUTIH, 25100 KUANTAN, PAHANG, MALAYSIA
  • KAHAIRI BIN ABDULLAH DEPARTMENT OF OTORHINOLARYNGOLOGY-HEAD & NECK SURGERY, KULLIYYAH OF MEDICINE, INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA (IIUM) PAHANG 25200, MALAYSIA
  • SITI AESAH @ NAZNIN BINTI MUHAMMAD DEPARTMENT OF PATHOLOGY. KULLIYYAH OF MEDICINE, INTERNATIONAL ISLAMIC UNIVERSITY MALAYSIA (IIUM), PAHANG 25200, MALAYSIA.

Abstract

The aim of this study was to investigate the level of miR-744 expression in nasopharyngeal carcinoma (NPC) tumour tissue and to provide initial clue on its potential as biomarkers for early detection of NPC in a preliminary analysis. Total miRNAs was extracted from NPC tissue as well as normal nasopharynx tissue taken from Hospital Tengku Ampuan Afzan (HTAA), Kuantan and converted into cDNA. The level of miR-744 expression in the cDNA was quantified using quantitative reverse transcription polymserase chain reaction (RT-qPCR) technique. The expression level of SNORD48 was measured simultaneously for each sample, which served as endogenous control. The difference in the expression of miR-744 in NPC and normal nasopharynx tissue were analysed using relative quantification, 2-ΔΔCT. In this preliminary analysis, this study found that miR-744 was upregulated in NPC as compared to normal nasopharynx tissue by 2.5 fold changes, respectively suggesting it may involve in progression of tumour. However, the finding is not significant and may not accurately reflect the overall population, due to small sample size involved in the study. Findings from the current study suggest the potential of miR-744 to serve as useful diagnostic and prognostic biomarker in NPC.

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Published

2018-07-05

How to Cite

KADERI, M. A. B., OTHMAN, N. S. B., LEMAN, W. I. B., ABDULLAH, K. B., & MUHAMMAD, S. A. @ N. B. (2018). THE EXPRESSION OF MICRORNA-744 (MIR-744) IN NASOPHARYNGEAL CARCINOMA: A PRELIMINARY STUDY. INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES, 2(1), 191–203. Retrieved from https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/113

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Original Articles