MODULATION OF THERMAL PROPERTIES OF BOVINE COLLAGEN SCAFFOLD USING VARIATION OF FABRICATION TEMPLATE

Authors

  • Nur Amnatasha Amerai Khan Department of Chemical Engineering and Sustainability, Kulliyyah of Engineering, IIUM
  • Munira Shahbuddin International Islamic University of Malaysia
  • Mohd Fauzi Busra 2Centre for Tissue Engineering & Regenerative Medicine, 12th Floor, Clinical Block Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia

Keywords:

collagen, DSC, scaffold, tissue engineering, matrix, biomaterials

Abstract

Tissue engineering and regenerative medicine are evolving towards utilising biomaterial or scaffold to regenerate tissue or organ. Scaffold plays a crucial role in the tissue or organ regeneration thus a study for the characterisation for the collagen scaffold need to be carried out. In this study, purified collagen was extracted from bovine tendon legs then the scaffold was fabricated using freeze drying method. The collagen scaffold was dehydrothermally treated and crosslinked with glutaraldehyde and followed by differential scanning calorimetry (DSC) analysis. From DSC analysis, the glass transition temperature, Tg for the sample in small pan with dimension 8.5cm×8.5cm×4cm is 60?C and 145?C for both without and with 1.0% glutaraldehyde crosslink respectively. The glass transition temperature for small pan is high which means that it is strong and rigid. Strong and rigid scaffold is needed in order to withstand the harsh condition within human body for the case of intestinal regeneration. Scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectroscopy are other techniques that can be used to further characterise collagen scaffold for its surface morphology also for its functional groups.

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References

I. V. Yannas, D. S. Tzeranis, B. A. Harley, and P. T. C. So, “Biologically active collagen-based scaffolds: Advances in processing and characterization,” Philos. Trans. R. Soc. A Math. Phys. Eng. Sci., vol. 368, no. 1917, pp. 2123–2139, 2010.

F. J. O. Brien and L. J. Gibson, “The effect of pore size on cell adhesion in collagen- GAG scaffolds .,” vol. 26, no. 4, 2005.

B. A. Harley and L. J. Gibson, “freeze-dried collagen-GAG scaffolds .,” vol. 25, no. 6, 2004.

E. Kontou and V. Kefalas, “Physical and chemical cross-linking effects in polyurethane elastomers,” no. July 1990, 2016.

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Published

2023-07-26

How to Cite

Amerai Khan, N. A., Shahbuddin, M., & Busra, . M. F. . (2023). MODULATION OF THERMAL PROPERTIES OF BOVINE COLLAGEN SCAFFOLD USING VARIATION OF FABRICATION TEMPLATE. Chemical and Natural Resources Engineering Journal (Formally Known As Biological and Natural Resources Engineering Journal), 7(1), 35–42. Retrieved from https://journals.iium.edu.my/bnrej/index.php/bnrej/article/view/83

Issue

Vol. 7 No. 1 (2023): VOL 7 NO 1 (2023): CHEMICAL AND NATURAL RESOURCES ENGINEERING JOURNAL (FORMALLY KNOWN AS BIOLOGICAL AND NATURAL RESOURCES ENGINEERING JOURNAL)

Section

Bioprocess Engineering

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