IDENTIFICATION OF GENETIC DIVERSITY AMONG BASIL (OCIMUM SP.) ACCESSIONS COLLECTED FROM AFRICA, EUROPE, ASIA, AND USA
Keywords:Basil, Genetic diversity, ISSR markers
Basil (Ocimum sp.), belonging to family Lamiaceae are very important for their therapeutic potentials and medicinal properties. In the present study, 62 basil accessions collected from different regions of the world were analysed for the assessment of genetic diversity using inter simple sequence repeat (ISSR). Four primers generated a total of 36 amplified fragments with an average of nine polymorphic bands per primer. The maximum number of fragments was produced by the primers 807 and 872 with 100% polymorphism, while the minimum number of fragments was produced by the primers 841 and 858 with 100% polymorphism. The highest similarity index was 0.59 and the least similarly index was 0.04. The weighted pair- group method was done using UPGMA clustering, classified the studied accessions in two major groups. It was concluded that high genetic variations were detected among the 62 Basil accessions. Based on the findings of this study, the ISSR markers are very useful for analysing the genetic variations for exploitation of basil in industrial applications.
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