OPTIMIZING FORMULATION AND SYNTHESIS CONDITIONS OF RED PALM OIL (RPO)-BASED NANOEMULSIONS STABILIZED BY TWEEN 80

Authors

  • Megat Nasir Megat Taufiq
  • Ricca Rahman Nasaruddin Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Jalan Gombak, 53100 Kuala Lumpur, Malaysia.
  • Mariatul Fadzillah Mansor

DOI:

https://doi.org/10.31436/cnrej.v9i2.129

Keywords:

Nanoemulsions, Red Palm Oil, Tween 80, High-speed homogenizer, Food application

Abstract

Nanoemulsion is an important class of nanomaterial that offers several advantages due to its improved stability and dispersibility in aqueous systems. In this preliminary study, oil-in-water nanoemulsions were synthesized using red palm oil (RPO). RPO was chosen for its high insoluble vitamin E content, a potent antioxidant that can reduce oxidative stress by neutralizing free radicals. The most suitable emulsifier, optimal formulation, and synthesis conditions to produce stable nanoemulsions were evaluated. A laser beam penetration test based on the Tyndall effect and light-scattering principles was used to confirm the presence of nanoemulsions qualitatively. The results indicate that Tween-80 produced the most stable and translucent nanoemulsion. The optimal formulation was found to have a weight ratio of 10:15:74:1 for RPO: Tween 80: water: glycerin. The optimal synthesis conditions were using the high-speed homogenizer at 15000 rpm for 40-minute synthesis time. The resulting nanoemulsions demonstrated stability suitable for further studies (e.g., physicochemical characterization, scale-up, and additional functionalization) for food and beverage applications.

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References

Singh T, Shukla S, Kumar P, Wahla V, Bajpai VK, Rather IA. (2017), Application of Nanotechnology in Food Science: Perception and Overview, Frontiers Microbiology, 8, 2017. https://doi.org/10.3389/fmicb.2017.01501

Carpenter J, Pinjari DV, Saharan VK, Pandit AB. (2022) Critical Review on Hydrodynamic Cavitation as an Intensifying Homogenizing Technique for Oil-in-Water Emulsification: Theoretical Insight, Current Status, and Future Perspectives. Industrial & Engineering Chemistry Research, 61(30), 10587-10602. https://doi.org/10.1021/acs.iecr.2c00754

Mason TG, Wilking JN, Meleson K, Chang CB, Graves SM. (2006) Nanoemulsions: formation, structure, and physical properties. Journal of Physics: Condensed Matter, 18, R635. doi: 10.1088/0953-8984/18/41/R01

Milin?i? DD, Salevi?-Jeli? AS, Kosti? AŽ, Stanojevi? SP, Nedovi? V, Peši? MB. (2023). Food nanoemulsions: how simulated gastrointestinal digestion models, nanoemulsion, and food matrix properties affect bio-accessibility of encapsulated bioactive compounds. Critical Reviews in Food Science and Nutrition, 64(22), 8091–8113. doi: 10.1080/10408398.2023.2195519

Ashaolu TJ. (2021) Nanoemulsions for health, food, and cosmetics: a review, Environmental Chemistry Letters, 19, 3381-3395. doi: 10.1007/s10311-021-01216-9

Roussell, M. (2015, February 27). Should you drink flavored water? Shape. Retrieved January 21, 2022, from https://www.shape.com/healthy-eating/healthy-drinks

Crintea A, Dutu AG, Sovrea, A, Constantin A-M, Samasca G, Masalar AL, Ifju B, Linga E, Neamti L, Tranca RA, Fekete Z, Silaghi CN, Cracium AM. (2022) Nanocarriers for Drug Delivery: An Overview with Emphasis on Vitamin D and K Transportation. Nanomaterials, 12, 1376. https://doi.org/10.3390/nano12081376

Aththar AF, Raushani F, Sekaringtyas FC, El-Muttaqien S, Setyawati DR, Pratiwi RD, Rifa’I M, Rahmawati SI, Putra MY, Ahmadi P, Indriani DW, Ling JTS, Widyastuti E, Bayu A, Rosyidah A. (2025) Palm oil nanoemulsion enhances tocotrienol stability, antioxidant, and selective anti-melanoma activity. Naunyn-Schmiedeberg's Archives Pharmacology, 2025. https://doi.org/10.1007/s00210-025-04296-4

Choi SJ, McClements DJ. (2020) Nanoemulsions as delivery systems for lipophilic nutraceuticals: strategies for improving their formulation, stability, functionality and bioavailability. Food Science Biotechnology, 29(2):149-168. doi: 10.1007/s10068-019-00731-4

Banasaz S, Morozova K, Ferrentino G, Scampicchio M. (2020) Encapsulation of Lipid-Soluble Bioactives by Nanoemulsions, Molecules, 31;25(17):3966. doi: 10.3390/molecules25173966

Yalçinöz ?, Erçelebi.(2020) Effect of surfactant type and droplet size on lipid oxidation in oil-in-water nano-emulsions, Quality Assurance and Safety of Crops & Foods, 12 (2). https://doi.org/10.15586/qas.v12i2.645

McClements DJ (2012) Nanoemulsions versus microemulsions: terminology, differences, and similarities. Soft Matter, 8, 1719-29. https://doi.org/10.1039/C2SM06903B

Azeem A, Rizwan M, Ahmad FJ, Iqbal Z, Khar RK, Aqil M, Talegaonkar S. (2009) Nanoemulsion components screening and selection: a technical note. AAPS PharmSciTech, 10(1):69-76. doi: 10.1208/s12249-008-9178-x

Kumar M, Bishnoi RS, Shukla AK, Jain CP. (2019) Techniques for Formulation of Nanoemulsion Drug Delivery System: A Review. Preventive Nutrition and Food Science, 24(3), 225-234. doi: 10.3746/pnf.2019.24.3.225

Souto EB, Cano A, Martins-Gomes C, Coutinho TE, Zieli?ska A, Silva AM. (2022) Microemulsions and Nanoemulsions in Skin Drug Delivery. Bioengineering (Basel), 9(4):158. doi: 10.3390/bioengineering9040158

Liu Q, Huang H, Chen H, Lin J, Wang Q. (2019) Food-Grade Nanoemulsions: Preparation, Stability and Application in Encapsulation of Bioactive Compounds. Molecules, 24(23), 4242. doi: 10.3390/molecules24234242

Published

2025-12-28

How to Cite

Megat Taufiq, M. N., Nasaruddin, R. R., & Mansor, M. F. (2025). OPTIMIZING FORMULATION AND SYNTHESIS CONDITIONS OF RED PALM OIL (RPO)-BASED NANOEMULSIONS STABILIZED BY TWEEN 80. Chemical and Natural Resources Engineering Journal (Formally Known As Biological and Natural Resources Engineering Journal), 9(2), 31–42. https://doi.org/10.31436/cnrej.v9i2.129