Biological Monitoring of Iodine Content in Human Breast Milk over Six Months Postpartum: A Case Study
DOI:
https://doi.org/10.31436/jop.v4i2.297Keywords:
Human milk, Iodine, ICP-MS, Acid digestion, PostpartumAbstract
Iodine deficiency was commonly reported in infants and partly attributable to low breast milk iodine content. The role of iodine is crucial in preventing brain damage and hypothyroidism in infants. It is important to monitor the concentration of iodine in breast milk of postpartum mothers. This study aimed to validate an analytical method to determine iodine concentration in human breast milk for biomonitoring purposes. Expressed breast milk samples were collected several times a day throughout six months postpartum from a healthy lactating mother. Samples were prepared with nitric acid digestion and analysed by inductively coupled plasma mass-spectrometry. Data analysis was conducted using Microsoft Excel 2016 for assessment of validation parameters and longitudinal concentration of iodine. The method validation parameters showed that linearity of calibration graph was 0.9987, limit of detection and limit of quantification were 0.218 µg/L and 0.661 µg/L, respectively. A recovery of 100.3% showed good accuracy, whereas inter-day and intra-day repeatability were 5.91% and 3.60%, respectively. The median iodine concentration was the highest in the first month (160.0 µg/L), then dropped to lower than recommended level (110 µg/L) from the second until six months postpartum (range: 31.9 - 98.7 µg/L). Fluctuation in median iodine concentration occurred over six months postpartum but circadian rhythm was observed to be consistent with “V” shaped curve pattern indicating higher concentration was exhibited in the morning and at night compared to evening. The analytical method was robust, accurate and reliable for measuring iodine concentration in human milk and applicable for biomonitoring. Deficiency in breast milk iodine content was observed in the second until six months postpartum. Iodine concentration in breast milk exhibited consistent circadian variation over six months postpartum.
References
Ballard, O., & Morrow, A. L. (2013). Human Milk Composition: Nutrients and Bioactive
Factors. Pediatric Clinics of North America, 60(1), 49–74.
https://doi.org/https://doi.org/10.1016/j.pcl.2012.10.002
Catenza, K. F., & Donkor, K. K. (2022). Determination of Heavy Metals in CannabinoidBased Food Products Using Microwave-Assisted Digestion and ICP-MS. Food
Analytical Methods, 15(9), 2537–2546. https://doi.org/10.1007/s12161-022-02315-1
Dror, D. K., & Allen, L. H. (2018). Iodine in human milk: A systematic review.
Advances in Nutrition, 9(10), 347S-357S. https://doi.org/10.1093/advances/nmy020
Eastman CJ, Zimmermann MB. (2018). The Iodine Deficiency Disorders. [Updated 2018
Feb 6]. In: Feingold KR, Anawalt B, Blackman MR, et al., editors. Endotext
[Internet]. South Dartmouth (MA): MDText.com, Inc.; 2000- . Available from:
https://www.ncbi.nlm.nih.gov/books/NBK285556/
Ellsworth, L., McCaffery, H., Harman, E., Abbott, J., & Gregg, B. (2020). Breast Milk Iodine
Concentration Is Associated with Infant Growth, Independent of Maternal Weight.
Nutrients, 12(2), 358. https://doi.org/10.3390/nu12020358
Fisher, W., Wang, J., George, N. I., Gearhart, J. M., & McLanahan, E. D. (2016).
Dietary iodine sufficiency and moderate insufficiency in the lactating mother and
nursing infant: A computational perspective. PLoS ONE, 11(3), 1–25.
https://doi.org/10.1371/journal.pone.0149300
Huang, C.-J., Li, J.-Z., Hwu, C.-M., Chen, H.-S., Wang, F.-F., Yeh, C.-C., Yang, C.-C.
(2023). Iodine Concentration in the Breast Milk and Urine as Biomarkers of Iodine
Nutritional Status of Lactating Women and Breastfed Infants in Taiwan. Nutrients,
:4125. https://doi.org/10.3390/nu15194125)
Mohd-Taufek, N. H., Mohmad Sabere, A. S., Mohamad Jamahari, U. S., Amran, N. B., Fata
Nahas, A. R., & Bidai, J. (2023). Determination of Zinc, Copper, Selenium, and
Manganese in Human Milk using Acid Digestion by ICP-MS and its Application in
Biological Trace Element Monitoring. Journal of Pharmacy, 3(2), 129-139
Mosca, F., & Giannì, M. L. (2017). Human milk: composition and health benefits.
Pediatria Medica e Chirurgica, 39(2). https://doi.org/10.4081/PMC.2017.155
Mulrine, H. M., Skeaff, S. A., Ferguson, E. L., Gray, A. R., & Valeix, P. (2010). Breast-milk
iodine concentration declines over the first 6 mo postpartum in iodine-deficient
women. American Journal of Clinical Nutrition, 92(4), 849–856.
https://doi.org/10.3945/ajcn.2010.29630
Nazeri, P., Dalili, H., Mehrabi, Y., Hedayati, M., Mirmiran, P., & Azizi, F. (2018). Breast Milk
Iodine Concentration Rather than Maternal Urinary Iodine Is a Reliable Indicator for
Monitoring Iodine Status of Breastfed Neonates. Biological Trace Element
Research, 185(1), 71–77. https://doi.org/10.1007/s12011-018-
-9
Peters, R., Li, B., Swinburn, B., Allender, S., He, Z., Lim, S. Y., Chea, M., Ding, G., Zhou,
W., Keonakhone, P., Vongxay, M., Khamphanthong, S., Selamat, R., Dayanghirang,
A., Abella, E., Da Costa, F., Chotivichien, S., Ungkanavin, N., Truong, M. T.,
Nguyen, S. D., … Poh, B. K. (2023). National nutrition surveillance programmes
in 18 countries in South-East Asia and Western Pacific Regions: a systematic
scoping review. Bulletin of the World Health Organization, 101(11), 690–706F.
https://doi.org/10.2471/BLT.23.289973
Selamat, R., Mohamud, W. N., Zainuddin, A. A., Rahim, N. S., Ghaffar, S. A., & Aris, T.
(2010). Iodine deficiency status and iodised salt consumption in Malaysia: findings
from a national iodine deficiency disorders survey. Asia Pacific journal of clinical
nutrition, 19(4), 578–585.
Ureta-Velasco, N., Keller, K., Escuder-Vieco, D., Serrano, J. C. E., García-Lara, N. R., &
Pallás-Alonso, C. R. (2022). Assessment of Iodine Concentration in Human Milk
from Donors: Implications for Preterm Infants. Nutrients, 14(20), 1–
https://doi.org/10.3390/nu14204304
Wei, R., Wang, Z., Zhang, X., Wang, X., Xu, Y., & Li, Q. (2023). Burden and trends of
iodine deficiency in Asia from 1990 to 2019. Public Health, 222, 75 – 84.
https://doi.org/10.1016/j.puhe.2023.06.034
Zhang, Y., Zhao, X., Shan, L., Jia, X., Liu, J., Gu, W., Zhang, Z., Zhang, X., & Sang,
Z. (2023). Variations in Breast Milk Iodine Concentration over 24 h among Lactating
Women in Northern China. Journal of Nutrition, 153(1), 208 – 214.
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