Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma

Authors

  • Lailatul Jalilah
  • Aziah Ismail
  • Norlelawati A. Talib
  • Naznin Muhammad
  • Faezahtul Arbaeyah Hussain
  • Norafiza Zainuddin

DOI:

https://doi.org/10.31436/imjm.v15i1.1218

Abstract

Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O 6 - guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation.

Downloads

Download data is not yet available.

Downloads

Published

01.06.2016

How to Cite

Jalilah, L. ., Ismail, A. ., A. Talib, N. ., Muhammad, N. ., Hussain, F. A. ., & Zainuddin, N. (2016). Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma. IIUM Medical Journal Malaysia, 15(1). https://doi.org/10.31436/imjm.v15i1.1218

Issue

Section

Supplementary Abstract

Most read articles by the same author(s)

<< < 1 2 3 > >>