OPTIMIZING THE HORMONAL CONCENTRATION FOR IN VITRO MATURATION OF BALB/c MICE OOCYTES AS A MAMMALIAN MODEL FOR CONSERVATION OF ENDANGERED SPECIES ANIMALS

Abstract

The conservation of endangered species is a global priority, with assisted reproductive technologies (ART) playing a key role in preserving biodiversity. This study investigates the potential of in vitro maturation (IVM) of oocytes to optimize IVM protocols for conservation efforts, using BALB/c mice as a model. The main objectives include determining the optimal dosages of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in IVM culture media and evaluating the effects of different IVM environments on oocyte quality. Oocytes were matured in M199 medium supplemented with lamb serum, FSH, LH, and penicillin-streptomycin, using varying FSH/LH ratios. A total of 360 oocytes were divided into four groups and incubated at 37°C for 24 hours. The results showed that 30IU concentrations of FSH and LH significantly improved oocyte maturation rates at 55.6%. Refining IVM protocols with this model provides a promising, less invasive tool for enhancing reproductive success and preserving genetic diversity in endangered species.

Keywords: In vitro maturation (IVM), hormonal manipulation, follicular stimulating hormone (FSH), luteinizing hormone (LH), oocyte grading.