A review of black seed extract as an agent in the wound healing process


  • Muhammad Hakeem Mohd Zaid Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia.
  • Muhammad Faezuddin Hanafi Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia
  • Muhammad Salahuddin Haris Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, 25200 Kuantan, Pahang, Malaysia.




Nigella Sativa, black seed, wound healing


Introduction: Nigella Sativa or Black Cumin is an annual flowering plant that can be used for wound treatment. In the treatment for wound healing, this plant contains many beneficial components that can help with the treatment but this plant utilisation in the medical field is still limited. This review will provide an overview of the advantages when this plant is used in the wound healing treatment.

Method: The review was based on the ROSES protocol, and the databases used were ScienceDirect, PubMed, and Google Scholar. After the search, only 11 papers had been chosen to be included in the results and they were divided into four main themes that were used for the analysis of the results.

Results: The themes were gross analysis, microscopic analysis, biochemical analysis and antimicrobial analysis.

Conclusion: In conclusion, the black seed extract contains several valuable properties, such as antimicrobial and antioxidant properties, which help improve the wound healing process. Further study needs to be done to discover more potential of Nigella Sativa in treating the wound.


Ab Rahman, M. R., Abdul Razak, F., & Mohd Bakri, M. (2014). Evaluation of Wound Closure Activity of Nigella sativa, Melastoma malabathricum, Pluchea indica, and Piper sarmentosum Extracts on Scratched Monolayer of Human Gingival Fibroblasts. Evidence-Based Complementary and Alternative Medicine, 2014, 1–9. https://doi.org/10.1155/2014/190342

Abd Elrahman, S. M., Younes, S. A., & Kawana, K. Y. (2019). Evaluation of Nigella Sativa on Socket Healing in Rabbits. Alexandria Dental Journal, 44(3), 60–64. https://doi.org/10.21608/adjalexu.2019.63559

Abu-Al-Basal M. A. (2011). Influence of Nigella sativa fixed oil on some blood parameters and histopathology of skin in staphylococcal-infected BALB/c mice. Pakistan journal of biological sciences: PJBS, 14(23), 1038–1046. https://doi.org/10.3923/pjbs.2011.1038.1046

Abuzinadah, O. (2009). Using Nigella sativa Oil to Treat and Heal Chemical Induced Wound of Rabbit Skin. Journal of King Abdulaziz University-science, 21, 335-346.

Ahmad, A., Husain, A., Mujeeb, M., Khan, S. A., Najmi, A. K., Siddique, N. A., Damanhouri, Z. A., & Anwar, F. (2013). A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5), 337–352. https://doi.org/10.1016/S2221-1691(13)60075-1

Ahmed, I. H., Awad, M. A., El-Mahdy, M., Gohar, H. M., & Ghanem, A. M. (1995). The effect of some medicinal plant extracts on wound healing in farm animals. Assiut Veterinary Medical Journal, 32(64), 236-244.

Al-Gaban, N. M., Ghani, B. A., & Kadhim, E. F. (2017). Evaluation of Topical Application of Propolis, Black Seeds, and Honey on Oral Mucosal Healing in Rabbits (Histological and Immunohistochemical Study on TGF-?3). International Journal of Science and Research (IJSR), 6(2), 1445–1450. https://doi.org/10.21275/ART20164558

Ali, A., Mohebbullah, M., Shahid, M. A., Alam, S., Uddin, M. N., Miah, M. S., Jamal, M. S. I., & Khan, M. S. (2020). PVA-Nigella Sativa nanofibrous mat: antibacterial efficacy and wound healing potentiality. Journal of the Textile Institute, 0(0), 1–11. https://doi.org/10.1080/00405000.2020.1831168

Apaydin, Y. B., & Gedikli, S. (2019). Wound healing effects of Nigella sativa L. essential oil in streptozotocin-induced in diabetic rats. GSC Biological and Pharmaceutical Sciences, 7(3), 030–039. https://doi.org/10.30574/gscbps.2019.7.3.0095

Cui, X., Gong, J., Han, H., He, L., Teng, Y., Tetley, T., Sinharay, R., Chung, K. F., Islam, T., Gilliland, F., Grady, S., Garshick, E., Li, Z., & Zhang, J. (2018). Relationship between free and total malondialdehyde, a well-established marker of oxidative stress, in various types of human biospecimens. Journal of Thoracic Disease, 10(5), 3088–3197. https://doi.org/10.21037/jtd.2018.05.92

Davies, J. E., & Hosseini, M. M. (2000). Histodynamics of endosseous wound healing. Bone engineering, 1(1), 1-14. Retrieved from https://www.researchgate.net/profile/John-Davies11/publication/255964870_Histodynamics_of_Endosseous_Wound_Healing/links/54dd0e120cf28a3d93f88e92/Histodynamics-of-Endosseous-Wound-Healing.pdf

Davoudi-Kiakalayeh, A., Mohammadi, R., Pourfathollah, A. A., Siery, Z., & Davoudi-Kiakalayeh, S. (2017). Alloimmunisation in thalassemia patients: New insight for healthcare. International Journal of Preventive Medicine, 8, 1–7. https://doi.org/10.4103/ijpvm.IJPVM

Dunnill, C., Patton, T., Brennan, J., Barrett, J., Dryden, M., Cooke, J., Leaper, D., & Georgopoulos, N. T. (2017). Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. International Wound Journal, 14(1), 89–96. https://doi.org/10.1111/iwj.12557

Dunnill, C., Patton, T., Brennan, J., Barrett, J., Dryden, M., Cooke, J., ... & Georgopoulos, N. T. (2017). Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS?modulating technologies for augmentation of the healing process. International wound journal, 14(1), 89-96.

Elgohary, H. M., Al Jaouni, S. K., & Selim, S. A. (2018). Effect of ultrasound-enhanced Nigella sativa seeds oil on wound healing: An animal model. Journal of Taibah University Medical Sciences, 13(5), 438–443. https://doi.org/10.1016/j.jtumed.2018.02.008

Etulain, J. Platelets in wound healing and regenerative medicine. Platelets. (2018), 29, 556–568. https://doi.org/10.1080/09537104.2018.1430357

Halawani, E. (2009). Antibacterial Activity of Thymoquinone and Thymohydroquinone of Nigella sativa L. and Their Interaction with Some Antibiotics. Advances in Biological Research, 3(56), 148–152.

Javadi, S. M. R., Hashemi, M., Mohammadi, Y., MamMohammadi, A., Sharifi, A., & Makarchian, H. R. (2018). Synergistic effect of honey and nigella sativa on wound healing in rats. Acta Cirurgica Brasileira, 33(6), 518–523. https://doi.org/10.1590/s0102-865020180060000006

Kumanda?, A., Karsli, B., Kürüm, A., Çinar, M., & Elma, E. (2020). Comparison of the effects of zinc-silver cream and nigella sativa oil on wound healing and oxidative stress in the wound model in rats. Ankara Universitesi Veteriner Fakultesi Dergisi, 67(1), 33–40. https://doi.org/10.33988/auvfd.563491

MacEwan, M. R., MacEwan, S., Kovacs, T. R., & Batts, J. (2017). What Makes the Optimal Wound Healing Material? A Review of Current Science and Introduction of a Synthetic Nanofabricated Wound Care Scaffold. Cureus, 9(10). https://doi.org/10.7759/cureus.1736

Mandal, A. M., Sene, P., & Manggang, R. K. J. (2015). A Review on Indian Medicinal Plants and Their Role in Wound Healing Activity. World Journal of Pharmaceutical Research, 4(6), 2204-2224.

Mastuti, S., Hadi, D. T., & Chodidjah, C. (2017). Nigella Sativa Seed Extract Ointment Increasing the Amount of Healing Fibroblast and Collagen on BALB/c Mice. Sains Medika, 8(1), 15. https://doi.org/10.26532/sainsmed.v8i1.1006

Mayet, N., Choonara, Y. E., Kumar, P., Tomar, L. K., Tyagi, C., Du Toit, L. C., & Pillay, V. (2014). A comprehensive review of advanced biopolymeric wound healing systems. Journal of pharmaceutical sciences, 103(8), 2211-2230. https://doi.org/10.1002/jps.24068

Mohammed, S. J., Amin, H. H. H., Aziz, S. B., Sha, A. M., Hassan, S., Abdul Aziz, J. M., & Rahman, H. S. (2019). Structural Characterization, Antimicrobial Activity, and in Vitro Cytotoxicity Effect of Black Seed Oil. Evidence-Based Complementary and Alternative Medicine, 2019. https://doi.org/10.1155/2019/6515671

Mirazi, N., Nourbar, E., Yari, S., Rafieian-Kopaei, M., & Nasri, H. (2019). Effect of hydroethanolic extract of Nigella sativa L. on skin wound healing process in diabetic male rats. International Journal of Preventive Medicine, 10(1), 18. https://doi.org/10.4103/ijpvm.ijpvm_276_18

Nourbar, E., Mirazi, N., Yari, S., Mahmoud, Rafieian?Kopaei, & Nasri, H. (2019). Effect of Hydroethanolic Extract of Nigella sativa L. on Skin Wound Healing Process in Diabetic Male Rats. International Journal of Preventive Medicine, 1–7. https://doi.org/10.4103/ijpvm.IJPVM

Rajabian, A., & Hosseinzadeh, H. (2020). Dermatological Effects of Nigella sativa and Its Constituent, Thymoquinone. In Nuts and Seeds in Health and Disease Prevention, 329-355. Elsevier Inc. https://doi.org/10.1016/b978-0-12-818553-7.00024-3

Razavi, B. M., & Hosseinzadeh, H. (2014). A review of the effects of Nigella sativa L. and its constituent, thymoquinone, in metabolic syndrome. Journal of Endocrinological Investigation, 37(11), 1031-1040.

Rohmah, D. I., & Suprayitno, E. (2020). Effect of Addition of Black Cumin Seed Oil (Nigella sativa) as a Source of Omega-6 on Cork Albumin Powder (Channa Striata) for Wound Closure. International Journal of Scientific and Research Publications (IJSRP), 10(7), 656–662. https://doi.org/10.29322/ijsrp.10.07.2020.p10371

Rowi?ski, R., Kozakiewicz, M., K?dziora-Kornatowska, K., Hübner-Wo?niak, E., & K?dziora, J. (2013). Markers of oxidative stress and erythrocyte antioxidant enzyme activity in older men and women with differing physical activity. Experimental Gerontology, 48(11), 1141-1146. https://doi.org/10.1016/j.exger.2013.07.010

Sallehuddin, N., Nordin, A., Idrus, R. B. H., & Fauzi, M. B. (2020). Nigella sativa and its active compound, thymoquinone, accelerate wound healing in an in vivo animal model: A comprehensive review. International Journal of Environmental Research and Public Health, 17(11), 1–17. https://doi.org/10.3390/ijerph17114160

Sahak, M. K. A., Kabir, N., Abbas, G., Draman, S., Hashim, N. H., & Hasan Adli, D. S. (2016). The Role of Nigella sativa and Its Active Constituents in Learning and Memory. Evidence-Based Complementary and Alternative Medicine, 2016, 1–6. https://doi.org/10.1155/2016/6075679

Sarkhail, P., Esmaily, H., Baghae, A., Shafiee, A., Abdollahi, M., & Sarkheil, P. (2011). Evaluation of Burn Healing Activity of Black Seed Oil in Rats. Planta Medica, 77(12), 34–39. https://doi.org/10.1055/s-0031-1282650

Sari, Y., Purnawan, I., Kurniawan, D. W., & Sutrisna, E. (2018). A comparative study of the effects of nigella sativa oil gel and aloe Vera gel on wound healing in diabetic rats. Journal of Evidence-Based Integrative Medicine, 23, 1–6. https://doi.org/10.1177/2515690X18772804

Selçuk, C. T., Durgun, M., Tekin, R., Yolbas, L., Bozkurt, M., Akçay, C., ... & Basarali, M. K. (2013). Evaluation of the effect of thymoquinone treatment on wound healing in a rat burn model. Journal of Burn Care & Research, 34(5), e274-e281. https://doi.org/10.1097/BCR.0b013e31827a2be1

Singh, S., Young, A., & McNaught, C.-E. (2017). The physiology of wound healing. Surgery (Oxford), 35(9), 473–477. https://doi.org/10.1016/j.mpsur.2017.06.004

Sinno, H., & Prakash, S. (2013). Complements and the Wound Healing Cascade: An Updated Review. Plastic Surgery International, 2013, 1–7. https://doi.org/10.1155/2013/146764

Trisnawati, R. (2018). The Effect of Thymoquinone Extract of Black Seeds Toward Socket New Blood Vessels Formation Process After Extraction in Diabetic-Induced Rats. In Research Article Journal of Diabetes and Cholesterol Metabolism (Vol. 2, Issue 1). DCM. Retrieved from http://rpdemos.net/clients/hendunadmin/admin/uploads/source/DCM/DCM-18-2-110.pdf

Turhan, Y. ?., Ar?can, M., Karaduman, Z. O., Turhal, O., Gams?zkan, M., Ayd?n, D., & Ozkan, K. (2019). Comparison of the Effects of Nigella sativa Oil and Nano-silver on Wound Healing in an Experimental Rat Model. Iranian Red Crescent Medical Journal, 10(10), 1259–1272. https://doi.org/10.5812/ircmj.84650.

Vigani, A., & Culler, C. A. (2017). Systemic and Local Management of Burn Wounds. Veterinary Clinics of North America: Small Animal Practice, 47(6), 1149–1163. https://doi.org/10.1016/j.cvsm.2017.06.003

Yaman, I., Durmus, A. S., Ceribasi, S., & Yaman, M. (2010). Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Veterinarni Medicina, 55(12), 619–624. https://doi.org/10.17221/2948-VETMED

Yimer, E. M., Tuem, K. B., Karim, A., Ur-Rehman, N., & Anwar, F. (2019). Nigella sativa L. (Black Cumin): A Promising Natural Remedy for Wide Range of Illnesses. Evidence-Based Complementary and Alternative Medicine, 2019, 1–16. https://doi.org/10.1155/2019/1528635

Yusmin, A., & Ahmad, N. (2017). Effect of thymoquinone on wound healing in alloxan-induced diabetic rats. Asian Journal of Pharmaceutical and Clinical Research, 10(9), 242–245. https://doi.org/10.22159/ajpcr.2017.v10i9.18951




How to Cite

Mohd Zaid, M. H., Hanafi, M. F., & Haris, M. S. (2021). A review of black seed extract as an agent in the wound healing process. Journal of Pharmacy, 1(2), 87–96. https://doi.org/10.31436/jop.v1i2.67



Original Articles