Effect of Trihoney (A Mixture of Trigona, Mellifera and Tualang) on Male Reproductive Hormones and Insulin Resistance in Hypercholesterolaemic Rabbits
DOI:
https://doi.org/10.31436/imjm.v19i3.1661Abstract
Introduction: Hypercholesterolaemia affects Sertoli and Leydig cells secretory functions, impairs steroid
hormone biosynthesis, and disrupts the hypothalamic-pituitary-gonadal axis. The use of honey in previous
studies resulted in an improvement of male reproductive hormonal disturbances. This study aimed to
investigate the protective effects of Trihoney on hypercholesterolemia-induced male reproductive hormonal
changes in male rabbits and compare its effects with atorvastatin. Materials and methods: Forty-eight male
New Zealand white rabbits were assigned into 6 groups as follows; Control: commercial pellet; CH:
commercial pellet with 0.6 g/kg/day Trihoney; HCD: 1% cholesterol diet; DH1: 1% cholesterol diet with 0.3
g/kg/day Trihoney; DH2: 1% cholesterol diet with 0.6 g/kg/day Trihoney and DAt: 1% cholesterol diet with
2mg/kg/day atorvastatin. After 12 weeks, the rabbits were sacrificed and blood samples were collected for
analysis of hormones and pro-inflammatory cytokines and calculation of HOMA-IR. The testes were
homogenized for intra-testicular testosterone measurement. Results: Serum testosterone reduced
significantly in HCD (p<0.05) and DAt (p<0.05) groups. Likewise, intra-testicular testosterone reduced
significantly in HCD (p<0.01) and DAt (p<0.01) groups. Serum FSH increased significantly in HCD (p<0.001)
and DAt (p<0.01). Trihoney particularly at the dose of 0.6 g/kg/day improved serum and intra-testicular
testosterone (p<0.05) and FSH (p<0.05). Trihoney and atorvastatin improved serum pro-inflammatory
cytokines. Trihoney and atorvastatin did not affect HOMA-IR. Conclusion: Trihoney attenuated the
detrimental effects of hypercholesterolaemia on male reproductive hormones which probably through a
local effect on testicular tissue and Trihoney anti-inflammatory effect independent of insulin resistance.
Atorvastatin did not counteract the impact of hypercholesterolaemia on the reproductive hormones.
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