Range Finding Test of Hydrocarbon on Scirpus mucronatus as Preliminary Test for Phytotoxicity of Contaminated Soil

Abstract

Abstract

Phytotoxicity is used to determine the maximum concentration of contaminant effect on plant growth in diesel fuel-contaminated soil. Range finding test is the first step that must be done to determine the concentration that the plant can survive in any contaminated soil. In this study, range finding test was conducted using Scirpus mucronatus to determine the maximum concentration of hydrocarbon that the species can survive. The test was conducted through a batch system, with each container containing media and three plants. The media used was spiked sand with dissolved diesel fuel in acetone of concentrations 0, 1, 5, 10, 15, 20, 50, 100, and 200 g/kg. Spiked sand was left for 2 weeks to homogenize the diesel and evaporate the acetone. Since the plants used were emergent plants, the containers contained 3L of de-ionized water. After 30 days of exposure, 33.3% of plants had withered in 5 and 10 g/kg; 66.6% in 15, 20, and 50 g/kg; and 100% in 100 and 200 g/kg of contaminant concentration. 

Keyword: phytotoxicity, range finding test, hydrocarbon, scirpus mucronatus

Abstrak

Ujian fitotoksin dilakukan untuk menentukan kesan konsentrasi maksimum pencemar terhadap tumbesaran tumbuhan di tanih yang dicemari minyak diesel. Ujian penentu julat dilakukan dahulu untuk menentukan tahap konsentrasi yang tidak menjejaskan kehidupan tumbuhan di tanih yang tercemar. Dalam kajian ini, ujian seperti ini dilakukan dengan menggunakan spesies Scirpus mucronatus untuk menentukan tahap maksimum hidrokarbon yang dapat ditahan. Ujian dilakukan mengikut sistem kelompok. Setiap bekas mengandungi media dan tiga tumbuhan. Media yang digunakan ialah tanih yang bercampur minyak diesel dalam aseton dengan konsentrasi 0, 1, 5, 10, 15, 20, 50, 100, and 200 g/kg. Tanih itu dibiarkan selama 2 minggu supaya diesel itu dapat dihomogen dan aseton itu sejat. Selepas didedahkan selama 30 hari, didapati 33.3% tumbuhan itu menjadi layu dalam konsentrasi 5 dan 10 g/kg, 66.6% bagi konsentrasi 15, 20, dan 50 g/kg, 100% bagi konsentrasi 100 dan 200 g/kg pencemar.

Kata kunci: fitotoksin, ujian penentu julat, hidrokarbon, Scirpus mucronatus