TY - JOUR AU - Arief Ichwan, Solachuddin Jauhari AU - Sazeli, Syahirah AU - Lestari, Widya PY - 2020/02/01 Y2 - 2024/03/28 TI - Analysis of the anti-cancer effect of ethyl-p-methoxycinnamate extracted cekur (Kaempferia galanga) on cancer cell lines with wild- type and null p53 JF - IIUM Journal of Orofacial and Health Sciences JA - IJOHS VL - 1 IS - 1 SE - Original Article DO - 10.31436/ijohs.v1i1.16 UR - https://journals.iium.edu.my/ktn/index.php/ijohs/article/view/16 SP - 28-33 AB - <p>This study aimed to examine the in-vitro anti-cancer potential of ethyl-p-methoxycinnamate (EPMC), the major constituent of <em>Kaempferia galanga</em> (<em>K. galanga</em>) in selected human lung adenocarcinoma cells line A549 (p53 wild-type) and H1299 (p53 null). The involvement of p53 pathway in the anti-cancer effect of EPMC on selected cells was determined using MTT assay and Real-time PCR. The MTT results show that EPMC induces cytotoxicity in a dose- dependent manner in A549 cancer cell lines containing the p53 wild-type gene. Meanwhile, our RT-PCR results indicate that the apoptotic activity of EPMC does not involve the p53 pathway. Overall, these results indicate that EPMC compounds of <em>K. galanga</em> stimulates in vitro cytotoxic and apoptotic activity unrelated to the p53 pathway</p> ER -