TY - JOUR AU - Haron, Ummi Aqilah AU - Abllah , Zurainie PY - 2020/01/15 Y2 - 2024/03/29 TI - Evaluation of Anti-Biofilm Activity of Virgin Coconut Oil (Avco) Against Streptococcus Mutans ATCC 2157 JF - IIUM Medical Journal Malaysia JA - imjm VL - 18 IS - 3 SE - DO - 10.31436/imjm.v18i2.634 UR - https://journals.iium.edu.my/kom/index.php/imjm/article/view/634 SP - AB - <p>Introduction: Dental caries has remained a major oral health problem worldwide.&nbsp; Streptococcus mutans&nbsp; is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth surface. In the previous study, the fatty acids profile of activated virgin coconut oil (AVCO) have been described, and it includes the excellent antimicrobial activity of the oil. The current study aims to evaluate the antibiofilm effect of AVCO against the&nbsp; Streptococcus mutans&nbsp; ATCC 2157 biofilm in vitro. Materials and method: The minimum biofilm inhibition concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) for antibiofilm activity were determined by serial dilution method, and biofilm thickness on&nbsp; S. mutans&nbsp; was quantified by confocal laser scanning microscopy (CLSM).&nbsp; Results:&nbsp; At the concentra-tion of 15.63 mg/ml of AVCO, it successfully inhibits the development of&nbsp; S. mutans&nbsp; ATCC 21575 biofilm and AVCO (62.52 mg/ml) eradicate biofilm that was formed by the bacteria. The z-stack images obtained from CLSM allows the construction of 3-D biofilm structure and a significant difference in the thickness of&nbsp; S. mutans&nbsp; ATCC 21575 biofilm pre and post-treatment with AVCO were observed.&nbsp; Conclusion:&nbsp; AVCO showed good potential as anticaries where it possesses the ability to inhibits and eradicate&nbsp; S. mutans&nbsp; ATCC 21575 biofilm. A future study to evaluates the interaction of individuals fatty acids present in AVCO against proteins that relate to biofilm formation of&nbsp; S. mutans&nbsp; can be performed utilising the molecular docking tools.</p> ER -