Burkholderia Pseudomallei Short-Chain  Dehydrogenase/ Oxidoreductase: Potential Urine  Biomarker Candidate for Acute Melioidosis

Aniza Pakeer; Vanitha  Mariappan; How Soon  Hin; Mohammed Imad A.  Mustafa Mahmud

doi:10.31436/imjm.v20i3.1699

Burkholderia Pseudomallei Short-Chain Dehydrogenase/ Oxidoreductase: Potential Urine Biomarker Candidate for Acute Melioidosis

Authors

Aniza Pakeer Dept. of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia Kuantan Campus, Pahang, Malaysia
Vanitha Mariappan Centre of Toxicology and Health Risk Studies (CORE), Faculty of Health Sciences, National University of Malaysia, Kuala Lumpur, Malaysia
How Soon Hin Dept. of Internal Medicine, Kulliyyah of Medicine, International Islamic University Malaysia Kuantan Campus, Pahang, Malaysia
Mohammed Imad A. Mustafa Mahmud Dept. of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia Kuantan Campus, Pahang, Malaysia

DOI:

https://doi.org/10.31436/imjm.v20i3.1699

Keywords:

Burkholderia pseudomalleI, biomarker, melioidosis, urine sample

Abstract

INTRODUCTION: In the current climate of urgency in identifying biomarkers for the development of rapid diagnostic kits, the use of urine samples to diagnose acute melioidosis was evaluated, comparing urine samples from Burkholderia pseudomallei culture-positive and culture-negative patients, and comparing pneumonic and septicemic melioidosis. MATERIAL AND METHODS: Eleven urine samples from clinically suspected melioidosis patients from a tertiary referral center, Hospital Tengku Ampuan Afzan, Pahang was used. An in-solution method for the detection of bacterial proteins using liquid chromatography-mass spectrometry quadrupole time-of-flight (LCMS QTOF) was used. RESULTS: Three bacterial proteins were consistently detected among all the culture[1]positive and PCR-positive cases tested, namely SDR family NAD(P)-dependent oxidoreductase protein (32kDa), 3-hydroxyacyl-CoA dehydrogenase Burkholderia sp. (32kDa), and NAD(P)-dependent dehydrogenase (short-subunit alcohol dehydrogenase family) Burkholderia sp. (33kDa). CONCLUSIONS: Short-chain dehydrogenase (SDO) proteins could potentially be a urine biomarker candidate as these have shown to aid in the ability of Burkholderia spp. to invade host cells as this action is important for the initial intracellular survival of the organism.

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Published

2021-07-01

How to Cite

Pakeer, A., Mariappan, V. ., Hin, H. S. ., & Mustafa Mahmud, M. I. A. . (2021). Burkholderia Pseudomallei Short-Chain Dehydrogenase/ Oxidoreductase: Potential Urine Biomarker Candidate for Acute Melioidosis. IIUM Medical Journal Malaysia, 20(3). https://doi.org/10.31436/imjm.v20i3.1699

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Issue

Vol. 20 No. 3 (2021): IIUM Medical Journal Malaysia - July 2021

Section

Original Articles

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