@article{HARON_MAT JUSOH_BT ABUL@ABD RAHMAN_2019, title={THE TOTAL PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF ROSELLE (Hibiscus sabdariffa) EXTRACT}, volume={3}, url={https://journals.iium.edu.my/ijahs/index.php/IJAHS/article/view/183}, DOI={10.31436/ijahs.v3i2.183}, abstractNote={<p><strong>Introduction:</strong> Roselle (<em>Hibiscus sabdariffa</em>) is widely known for its benefits and medicinal properties. The aim of this study was to determine the total phenolic content (TPC) in <em>H. sabdariffa</em> extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity. <strong>Methods:</strong> The TPC value for each phenolic extract in free, bound soluble and bound insoluble was determined by using Folin-Ciocalteu assay. The antioxidant scavenging capacity was evaluated by using DPPH assay. <strong>Results:</strong> The results obtained in this study showed that bound soluble phenolic compound (7.63 <u>+</u> 0.28 mg GAE/g DW) had the highest TPC value than the free and insoluble phenolic compound extracts (2.78 <u>+</u> 0.20 mg GAE/g DW and 3.61 <u>+</u> 0.85 mg GAE/g DW, respectively) (p<0.05). As for the IC<sub>50</sub> values, bound soluble extract exhibited the greatest value(0.62%) as compared to the free and bound insoluble phenolic extracts  (0.90% and 1.14%, respectively). A positive correlation (R<sup>2</sup> = 0.956) between three fraction (free, soluble and insoluble) of TPC and antioxidant radical scavenging capacity (DPPH) was obtained.  <strong>Conclusion: </strong>It can be concluded that phenolic compound in <em>H. sabdariffa</em> extracts have an effect to the antioxidant scavenging capacity particularly in the bound soluble form. This finding proved that <em>H. sabdariffa</em> extract could be a good source of natural antioxidant for food, cosmetic and pharmaceutical industry.</p>}, number={2}, journal={INTERNATIONAL JOURNAL OF ALLIED HEALTH SCIENCES}, author={HARON, NORMAH and MAT JUSOH, HANAPI and BT ABUL@ABD RAHMAN, NUR SYUHAIDA}, year={2019}, month={Aug.}, pages={725–733} }