ANTI-PROLIFERATIVE PROPERTY OF PORCUPINE BEZOAR EXTRACT ON MELANOMA (A375)
Porcupine bezoar (PB) has been previously reported to possess various medicinal properties. However, its potential as an anticancer agent against human cancers is still poorly studied and understood. Hence, in this study, porcupine bezoar (PB) aqueous extract was evaluated for its potential as a safe anticancer agent. Initially PB was infused in water for 18 h to get PB aqueous extract which was preliminary tested for total phenolic content (TPC), total flavonoid content (TFC), DPPH activity in order to confirm the presence of bioactive agents as antioxidants in PB. Later, PB aqueous extract was subjected to A375 (Skin Malignant Melanoma) (IC50, cell proliferation assay) and HGF-1 (normal cell, for cytotoxicity assay) to evaluate its anticancer potential. PB aqueous extract showed 11.68 μg/mL ± 0.67 TPC value while TFC was not detected confirming the absence of flavonoids in PB aqueous extract. IC50 for DPPH was found to be 0.79 μg/mL ± 0.07. The IC50 result for A375 was found to be 10.1 µg/mL ± 0.17. PB aqueous extract showed significant anti-proliferation pattern at 24, 48 and 72 hours exposures. The data from this study suggest the potential alternative use of PB in developing natural antioxidants and anti-proliferative agents for improving human health.