BIOELECTROCHEMICAL BEHAVIOR OF WILD TYPE BACILLUS CEREUS IN DUAL CHAMBER MICROBIAL FUEL CELL
DOI:
https://doi.org/10.31436/iiumej.v18i2.801Abstract
Microbial fuel cell (MFC) is a bioelectrochemical system that uses bacteria as biocatalyst to oxidize organic substrates as well as release electrons, which can be harvested in an external circuit to produce electrical energy. In this study, a proteolytic biocatalyst Bacillus cereus (B. cereus) has been employed for the first time in a microbial fuel cell (MFC). The wild type pure culture was isolated from municipal wastewater and identified using Biolog Gen III analysis. The MFCs were fueled with palm oil mill effluent (POME) and attained the maximum power density of about 3.88 W/m3. The electrochemical behavior of MFC operated by B. cereus was evaluated using polarization curve, electrochemical impedance spectroscopy (EIS) and cyclic voltammetery (CV) analysis. B. Cereus excreated electron shuttling compound which significantly reduced the anode charge transfer resistance (52.95%). The FESEM result shows that B. Cereus has the capability of effective biofilm formation. These results revealed the electrocatalytic potentiality of B. cereus and which makes it a promising candidate to be used in MFCs. Therfore, this biocatlyst can be used to generate electricity through the wastewater valorization.Downloads
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[2] Z. Z. Ismail, and A. J. Jaeel. "Sustainable power generation in continuous flow microbial fuel cell treating actual wastewater: Influence of Biocatalyst Type on Electricity Production," The Scientific World Journal. pp. 1-7, 2013.
[3] M. A. Islam, M. R. Khan, A. Yousuf, W. C. Wai, and C. K. Cheng. "Electricity generation form pretreated palm oil mill effluent using Klebsiella Variicola as an inoculum in Microbial fuel cell," 4th International Conference on the Development in the in Renewable Energy Technology (ICDRET), Proc. 1-4, 2016.
[4] B. E. Logan, and J. M. Regan. "Microbial fuel cells-challenges and applications," Environ. Sci. Technol. Vol. 40, pp. 5172-5180, 2006.
[5] D. Majumder, J. P. Maity, M. J. Tseng, V. R. Nimje, H. R. Chen, C. C. Chen, Y. F. Chang, T. C. Yang, and C. Y. Chen. "Electricity generation and wastewater treatment of oil refinery in microbial fuel cells using Pseudomonas putida," Int J Mol Sci. Vol. 15, pp. 16772-16786, 2014.
[6] N. Li, L. Liu, and F. Yang. "Power generation enhanced by a polyaniline–phytic acid modified filter electrode integrating microbial fuel cell with membrane bioreactor," Sep. Purif. Technol. Vol. 132, pp. 213-217, 2014.
[7] S. T. Read, P. Dutta, P. L. Bond, J. Keller and K. Rabaey. "Initial development and structure of biofilms on microbial fuel cell anodes," BMC Microbiol. Vol. 10, 2010.
[8] V. R. Nimje, C. -Y. Chen, C. -C. Chen, J. -S. Jean, A .S. Reddy, C. W. Fan, K. Y. Pan, H. T. Liu, and J. L. Chen. "Stable and high energy generation by a strain of Bacillus subtilis in a microbial fuel cell," J. Power Sources. Vol. 190, pp. 258-263, 2009.
[9] A. E. Vélez-Lee, F. Cordova-Lozano, E. R. Bandala, and J. L. Sanchez-Salas. "Cloning and expression of vgb gene in Bacillus cereus, improve phenol and p-nitrophenol biodegradation," Physics and Chemistry of the Earth. Vol. 91, pp. 38-45, 2016.
[10] M. A. Islam, W. W. Chee, E. Baranitharan, Y. Abu, K. C. Chin and M. R. Khan. "Evaluation of Electricity Generation and Wastewater Treatment from Palm Oil Mill Effluent Using Single and Dual Chamber microbial fuel cell," National Ponference for Postgraduate Research, Proc. 100-106, 2016.
[11] A. Banerjee, and A. K. Ghoshal. "Phenol degradation by Bacillus cereus: pathway and kinetic modeling," Bioresour. Technol. Vol. 101, pp. 5501-5507, 2010.
[12] L. Deng, F. Li, S. Zhou, D. Huang, and J. Ni. "A study of electron-shuttle mechanism in Klebsiella pneumoniae based-microbial fuel cells," Chin. Sci. Bull. Vol. 55, pp. 99-104, 2010.
[13] K. C. Wrighton, P. Agbo, F. Warnecke, K. A. Weber, E. L. Brodie, T. Z. DeSantis, P. Hugenholtz, G. L. Andersen, and J. D. Coates. "A novel ecological role of the Firmicutes identified in thermophilic microbial fuel cells," The ISME journal. Vol. 2, pp. 1146-1156, 2008.
[14] E. Marsili, D. B. Baron, I. D. Shikhare, D. Coursolle, J. A. Gralnick, and D. R. Bond. Shewanella secretes flavins that mediate extracellular electron transfer," Proceedings of the National Academy of Sciences, Vol. 105, Proc. 3968-3973, 2008.
[15] A. Nandy, V. Kumar, and P. P. Kundu, "Utilization of proteinaceous materials for power generation in a mediatorless microbial fuel cell by a new electrogenic bacteria Lysinibacillus sphaericus VA5," Enzyme Microb. Technol. Vol. 53, pp. 339-344, 2013.
[16] E. Baranitharan, M. R. Khan, A. Yousuf, W. F. A. Teo, G. Y. A. Tan, and C. K. Cheng. "Enhanced power generation using controlled inoculum from palm oil mill effluent fed microbial fuel cell," Fuel. Vol. 143, pp. 72-79, 2015.
[17] M. A. Islam, M. Rahman, A. Yousuf, C. K. Cheng and C. W. Woon. "Performance of Klebsiella oxytoca to generate electricity from POME in microbial fuel cell," MATEC Web of Conferences, Vol. 38, Proc. 1-6, 2016.
[18] R. P. Ramasamy, Z. Ren, M. M. Mench, and J. M. Regan. "Impact of initial biofilm growth on the anode impedance of microbial fuel cells," Biotechnol. Bioeng. Vol. 101, pp. 101-108, 2008.
[19] E. Marsili, J. B. Rollefson, D. B. Baron, R. M. Hozalski, and D. R. Bond. "Microbial biofilm voltammetry: direct electrochemical characterization of catalytic electrode-attached biofilms," Applied and environmental microbiology. Vol. 74, pp. 7329-7337, 2008.
[20] Z. He, and F. Mansfeld. "Exploring the use of electrochemical impedance spectroscopy (EIS) in microbial fuel cell studies," Energy Environ. Sci. Vol. 2, pp. 215-219, 2009.
[21] N. Samsudeen, T. Radhakrishnan, and M. Matheswaran. "Bioelectricity production from microbial fuel cell using mixed bacterial culture isolated from distillery wastewater," Bioresour. Technol. Vol. 195, pp. 242-247, 2015.
[22] M. A. Islam, C. W. Woon, B. Ethiraj, C. K. Cheng, A. Yousuf and M. M. R. Khan. "Ultrasound driven biofilm removal for stable power generation in microbial fuel cell," Energy Fuels. 2016.
