PURIFICATION AND PARTIAL CHARACTERIZATION OF L-ASPARAGINASE ENZYME PRODUCED BY NEWLY ISOLATE BACILLUS SP.

  • Dzun Noraini Jimat International Islamic University Malaysia
  • Intan Baizura Firda Mohamed International Islamic University Malaysia
  • Azlin Suhaida Azmi International Islamic University Malaysia
  • Parveen Jamal International Islamic University Malaysia

Abstract

A newly bacterial producing L-asparaginase was successful isolated from Sungai Klah Hot Spring, Perak, Malaysia and identified as Bacillus sp. It was the best L-asparaginase producer as compared to other isolates. Production of L-asparaginase from the microbial strain was carried out under liquid fermentation. The crude enzyme was then centrifuged and precipitated with ammonium sulfate before further purified with chromatographic method. The ion exchange chromatography HiTrap DEAE-Sepharose Fast Flow column followed by separation on Superose 12 gel filtration were used to obtain pure enzyme. The purified enzyme showed 10.11 U/mg of specific activity, 50.07% yield with 2.21 fold purification. The purified enzyme was found to be dimer in form, with a molecular weight of 65 kDa as estimated by SDS-PAGE. The maximum activity of the purified L-asparaginase was observed at pH 9 and temperature of 60°C.

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Author Biographies

Dzun Noraini Jimat, International Islamic University Malaysia
Department of Biotechnology Engineering
Intan Baizura Firda Mohamed, International Islamic University Malaysia
Department of Biotechnology Engineering
Azlin Suhaida Azmi, International Islamic University Malaysia
Department of Biotechnology Engineering
Parveen Jamal, International Islamic University Malaysia
Department of Biotechnology Engineering

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Published
2017-12-01
How to Cite
Jimat, D. N., Mohamed, I. B. F., Azmi, A. S., & Jamal, P. (2017). PURIFICATION AND PARTIAL CHARACTERIZATION OF L-ASPARAGINASE ENZYME PRODUCED BY NEWLY ISOLATE BACILLUS SP. IIUM Engineering Journal, 18(2), 1-10. https://doi.org/10.31436/iiumej.v18i2.654
Section
Chemical and Biotechnology Engineering